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J Mol Evol
1988 Jan 01;271:17-28. doi: 10.1007/bf02099726.
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Cytoskeletal actin gene families of Xenopus borealis and Xenopus laevis.
Cross GS
,
Wilson C
,
Erba HP
,
Woodland HR
.
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We have sequenced the coding and leader regions, as well as part of the 3' untranslated region, of a Xenopus borealis type 1 cytoskeletal actin gene [defined according to the arrangement of acidic residues at the N-terminus; Vandekerckhove et al. (1981) J Mol Biol 152:413-426]. The encoded amino acid sequence is the same as the avian and mammalian beta (type 1) cytoskeletal actins, except for an isoleucine at position 10 (as found in the mammalian gamma cytoskeletal actins), and an extra amino acid, alanine, after the N-terminal methionine. Five introns were found, in the same positions as those of the rat and chicken beta-actin genes. The 5' and 3' untranslated regions resemble those of the human gamma (type 8) cytoskeletal actin gene more closely than the mammalian beta genes. Primer extension showed that this type 1 gene is transcribed in ovary and tadpole. Sequencing of primer extension products demonstrated two additional mRNA species in X. borealis, encoding type 7 and 8 isoforms. This contrasts with the closely related species Xenopus laevis, where type 4, 5, and 8 isoforms have been found. The type 7 isoform has not previously been found in any other species. The mRNAs of the X. borealis type 1 and 8 and X. laevis type 5 and 8 isoforms contain highly homologous leaders. The X. borealis type 7 mRNA has no leader homology with the other mRNA species and, unlike them, has no extra N-terminal alanine codon. The evolutionary implications of these data are discussed.
Ballantine,
Changes in protein synthesis during the development of Xenopus laevis.
1979, Pubmed,
Xenbase
Ballantine,
Changes in protein synthesis during the development of Xenopus laevis.
1979,
Pubmed
,
Xenbase
Bender,
The structure of M6, a recombinant plasmid containing Dictyostelium DNA homologous to actin messenger RNA.
1978,
Pubmed
Benoist,
The ovalbumin gene-sequence of putative control regions.
1980,
Pubmed
Benton,
Screening lambdagt recombinant clones by hybridization to single plaques in situ.
1977,
Pubmed
Bergsma,
Novel chicken actin gene: third cytoplasmic isoform.
1985,
Pubmed
Bisbee,
Albumin phylogeny for clawed frogs (Xenopus).
1977,
Pubmed
,
Xenbase
Breathnach,
Organization and expression of eucaryotic split genes coding for proteins.
1981,
Pubmed
Bremer,
Evidence for a species of nuclear actin distinct from cytoplasmic and muscles actins.
1981,
Pubmed
Chang,
The complete sequence of the chicken alpha-cardiac actin gene: a highly conserved vertebrate gene.
1985,
Pubmed
Cooper,
Complete nucleotide sequence of a sea urchin actin gene.
1982,
Pubmed
Efstratiadis,
The structure and evolution of the human beta-globin gene family.
1980,
Pubmed
Erba,
Nucleotide sequence of the human gamma cytoskeletal actin mRNA: anomalous evolution of vertebrate non-muscle actin genes.
1986,
Pubmed
Files,
Actin gene family of Caenorhabditis elegans.
1983,
Pubmed
Fornwald,
The complete nucleotide sequence of the chick a-actin gene and its evolutionary relationship to the actin gene family.
1982,
Pubmed
Fyrberg,
The actin genes of Drosophila: protein coding regions are highly conserved but intron positions are not.
1981,
Pubmed
Gunning,
Evolution of the human sarcomeric-actin genes: evidence for units of selection within the 3' untranslated regions of the mRNAs.
1984,
Pubmed
,
Xenbase
Hamada,
Molecular structure and evolutionary origin of human cardiac muscle actin gene.
1982,
Pubmed
Hanauer,
Isolation and characterization of cDNA clones for human skeletal muscle alpha actin.
1983,
Pubmed
Kost,
The nucleotide sequence of the chick cytoplasmic beta-actin gene.
1983,
Pubmed
Luse,
Transcription of the beta-like globin genes and pseudogenes of the goat in a cell-free system.
1981,
Pubmed
Marotta,
Microheterogeneity of brain cytoplasmic and synaptoplasmic actins.
1978,
Pubmed
Maxam,
Sequencing end-labeled DNA with base-specific chemical cleavages.
1980,
Pubmed
Mayer,
Expression of the genes coding for the skeletal muscle and cardiac actions in the heart.
1984,
Pubmed
Miller,
c-fos protein can induce cellular transformation: a novel mechanism of activation of a cellular oncogene.
1984,
Pubmed
Nudel,
The nucleotide sequence of the rat cytoplasmic beta-actin gene.
1983,
Pubmed
Ordahl,
Strong homology in promoter and 3'-untranslated regions of chick and rat alpha-actin genes.
1983,
Pubmed
Ponte,
Evolutionary conservation in the untranslated regions of actin mRNAs: DNA sequence of a human beta-actin cDNA.
1984,
Pubmed
Pudney,
Establishment of a cell line (XTC-2) from the South African clawed toad, Xenopus laevis.
1973,
Pubmed
,
Xenbase
Sanchez,
Two Drosophila actin genes in detail. Gene structure, protein structure and transcription during development.
1983,
Pubmed
Sanger,
Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing.
1980,
Pubmed
Schuler,
DNA sequence of two linked actin genes of sea urchin.
1983,
Pubmed
Shah,
Complete nucleotide sequence of a soybean actin gene.
1982,
Pubmed
Smith,
A simple method for DNA restriction site mapping.
1976,
Pubmed
Southern,
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
1975,
Pubmed
Stutz,
Isolation and characterization of sarcomeric actin genes expressed in Xenopus laevis embryos.
1986,
Pubmed
,
Xenbase
Thiébaud,
DNA content in the genus Xenopus.
1977,
Pubmed
,
Xenbase
Tymowska,
Chromosome complements of the genus Xenopus.
1973,
Pubmed
,
Xenbase
Ueyama,
Structure of a human smooth muscle actin gene (aortic type) with a unique intron site.
1984,
Pubmed
Vandekerckhove,
Actin typing on total cellular extracts: a highly sensitive protein-chemical procedure able to distinguish different actins.
1981,
Pubmed
Vandekerckhove,
Chordate muscle actins differ distinctly from invertebrate muscle actins. The evolution of the different vertebrate muscle actins.
1984,
Pubmed
Vandekerckhove,
Actin amino-acid sequences. Comparison of actins from calf thymus, bovine brain, and SV40-transformed mouse 3T3 cells with rabbit skeletal muscle actin.
1978,
Pubmed
Vandekerckhove,
Diversity of expression of non-muscle actin in amphibia.
1981,
Pubmed
,
Xenbase
Vandekerckhove,
Mammalian cytoplasmic actins are the products of at least two genes and differ in primary structure in at least 25 identified positions from skeletal muscle actins.
1978,
Pubmed
Vandekerckhove,
At least six different actins are expressed in a higher mammal: an analysis based on the amino acid sequence of the amino-terminal tryptic peptide.
1978,
Pubmed
Vandekerckhove,
The complete amino acid sequence of actins from bovine aorta, bovine heart, bovine fast skeletal muscle, and rabbit slow skeletal muscle. A protein-chemical analysis of muscle actin differentiation.
1979,
Pubmed
Wilson,
Biochemical evolution.
1977,
Pubmed
Woodland,
Are there major developmentally regulated H4 gene classes in Xenopus?
1984,
Pubmed
,
Xenbase
Yaffe,
Highly conserved sequences in the 3' untranslated region of mRNAs coding for homologous proteins in distantly related species.
1985,
Pubmed
Zakut,
Nucleotide sequence of the rat skeletal muscle actin gene.
1982,
Pubmed
