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EMBO J
1987 Oct 01;610:3071-8. doi: 10.1002/j.1460-2075.1987.tb02614.x.
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Functional characterization of X. laevis U5 snRNA genes.
Kazmaier M
,
Tebb G
,
Mattaj IW
.
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Xenopus laevis U5 snRNA genes are found in several genomic arrangements, represented by a predominant tandem repeat of 583 bp and other minor repeats. Several copies of the major tandem repeat have been cloned and expressed in Xenopus oocytes. The transcripts assemble into U5 snRNPs which are recognized by anti-Sm antibodies. We have identified functional elements in the U5 gene promoter. Although similar in organization to other U snRNA gene promoters, U5 contains significant differences and is more efficiently expressed than the Xenopus U2 gene in oocytes. The proximal sequence element (PSE), although homologous to a mammalian consensus for this region (Skuzeski et al., 1984), does not resemble the previously characterized Xenopus U1 and U2 PSEs closely in sequence. The ATGCAAAT (octamer) part of the distal sequence element (DSE 1) is found in U5 in the orientation opposite to that in U1 and U2 gene promoters. DNase I protection experiments led to the identification of a third element (DSE 2), situated close to the octamer motif. Analysis of deletion mutants showed that both DSE 1 and 2 are essential parts of the U5 gene enhancer, and provides evidence that U snRNA enhancers are complex structures consisting of more than one site of DNA-factor interaction.
Ares,
Orientation-dependent transcriptional activator upstream of a human U2 snRNA gene.
1985, Pubmed,
Xenbase
Ares,
Orientation-dependent transcriptional activator upstream of a human U2 snRNA gene.
1985,
Pubmed
,
Xenbase
Berk,
Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids.
1977,
Pubmed
Bohmann,
A transcription factor which binds to the enhancers of SV40, immunoglobulin heavy chain and U2 snRNA genes.
,
Pubmed
,
Xenbase
Branlant,
High evolutionary conservation of the secondary structure and of certain nucleotide sequences of U5 RNA.
1983,
Pubmed
Busch,
SnRNAs, SnRNPs, and RNA processing.
1982,
Pubmed
Ciliberto,
Transcription signals in embryonic Xenopus laevis U1 RNA genes.
1985,
Pubmed
,
Xenbase
Ciliberto,
Formation of the 3' end on U snRNAs requires at least three sequence elements.
1986,
Pubmed
,
Xenbase
Ciliberto,
Properties of a U1 RNA enhancer-like sequence.
1987,
Pubmed
,
Xenbase
De Robertis,
Intracellular transport of microinjected 5S and small nuclear RNAs.
1982,
Pubmed
,
Xenbase
Dignam,
Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.
1983,
Pubmed
Falkner,
Correct transcription of an immunoglobulin kappa gene requires an upstream fragment containing conserved sequence elements.
,
Pubmed
Fritz,
Small nuclear U-ribonucleoproteins in Xenopus laevis development. Uncoupled accumulation of the protein and RNA components.
1984,
Pubmed
,
Xenbase
Galas,
DNAse footprinting: a simple method for the detection of protein-DNA binding specificity.
1978,
Pubmed
Hernandez,
Formation of the 3' end of U1 snRNA is directed by a conserved sequence located downstream of the coding region.
1985,
Pubmed
Hernandez,
Formation of the 3' end of U1 snRNA requires compatible snRNA promoter elements.
1986,
Pubmed
Hoffman,
Structural and functional analysis of chicken U4 small nuclear RNA genes.
1986,
Pubmed
,
Xenbase
Jacob,
A family of small nucleoplasmic RNAs with common structural features.
1984,
Pubmed
,
Xenbase
Krol,
The two embryonic U1 RNA genes of Xenopus laevis have both common and gene-specific transcription signals.
1985,
Pubmed
,
Xenbase
Krol,
Xenopus tropicalis U6 snRNA genes transcribed by Pol III contain the upstream promoter elements used by Pol II dependent U snRNA genes.
1987,
Pubmed
,
Xenbase
Kunkel,
U6 small nuclear RNA is transcribed by RNA polymerase III.
1986,
Pubmed
Lerner,
Antibodies to small nuclear RNAs complexed with proteins are produced by patients with systemic lupus erythematosus.
1979,
Pubmed
Lund,
The two embryonic U1 small nuclear RNAs of Xenopus laevis are encoded by a major family of tandemly repeated genes.
1984,
Pubmed
,
Xenbase
Madore,
Intracellular site of U1 small nuclear RNA processing and ribonucleoprotein assembly.
1984,
Pubmed
Mangin,
Human U2 small nuclear RNA genes contain an upstream enhancer.
1986,
Pubmed
,
Xenbase
Maniatis,
The role of small nuclear ribonucleoprotein particles in pre-mRNA splicing.
,
Pubmed
Mattaj,
Cap trimethylation of U snRNA is cytoplasmic and dependent on U snRNP protein binding.
1986,
Pubmed
,
Xenbase
Mattaj,
Xenopus laevis U2 snRNA genes: tandemly repeated transcription units sharing 5' and 3' flanking homology with other RNA polymerase II transcribed genes.
1983,
Pubmed
,
Xenbase
Mattaj,
Nuclear segregation of U2 snRNA requires binding of specific snRNP proteins.
1985,
Pubmed
,
Xenbase
Mattaj,
An enhancer-like sequence within the Xenopus U2 gene promoter facilitates the formation of stable transcription complexes.
,
Pubmed
,
Xenbase
Maxam,
Sequencing end-labeled DNA with base-specific chemical cleavages.
1980,
Pubmed
Messing,
A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments.
1982,
Pubmed
Minowada,
Rosette-forming human lymphoid cell lines. I. Establishment and evidence for origin of thymus-derived lymphocytes.
1972,
Pubmed
Murphy,
Functional elements of the human U1 RNA promoter. Identification of five separate regions required for efficient transcription and template competition.
1987,
Pubmed
,
Xenbase
Murphy,
Transcription of a gene for human U1 small nuclear RNA.
1982,
Pubmed
,
Xenbase
Nishikura,
Genetic analysis of the processing of a spliced tRNA.
1982,
Pubmed
,
Xenbase
Parslow,
Structure of the 5' ends of immunoglobulin genes: a novel conserved sequence.
1984,
Pubmed
Reddy,
The capped U6 small nuclear RNA is transcribed by RNA polymerase III.
1987,
Pubmed
,
Xenbase
Sanger,
DNA sequencing with chain-terminating inhibitors.
1977,
Pubmed
Schaufele,
Compensatory mutations suggest that base-pairing with a small nuclear RNA is required to form the 3' end of H3 messenger RNA.
,
Pubmed
,
Xenbase
Sharp,
Splicing of messenger RNA precursors.
1987,
Pubmed
Singh,
A nuclear factor that binds to a conserved sequence motif in transcriptional control elements of immunoglobulin genes.
,
Pubmed
Sive,
Interaction of a common factor with conserved promoter and enhancer sequences in histone H2B, immunoglobulin, and U2 small nuclear RNA (snRNA) genes.
1986,
Pubmed
Skuzeski,
Synthesis of human U1 RNA. II. Identification of two regions of the promoter essential for transcription initiation at position +1.
1984,
Pubmed
,
Xenbase
Strub,
Genetic complementation in the Xenopus oocyte: co-expression of sea urchin histone and U7 RNAs restores 3' processing of H3 pre-mRNA in the oocyte.
1986,
Pubmed
,
Xenbase
Westin,
Human U2 and U1 RNA genes use similar transcription signals.
1984,
Pubmed
,
Xenbase
Wildeman,
Stimulation of in vitro transcription from the SV40 early promoter by the enhancer involves a specific trans-acting factor.
1984,
Pubmed
Yuo,
Sequences required for 3' end formation of human U2 small nuclear RNA.
1985,
Pubmed
,
Xenbase
Zeller,
Xenopus laevis U1 snRNA genes: characterisation of transcriptionally active genes reveals major and minor repeated gene families.
1984,
Pubmed
,
Xenbase
de Vegvar,
3' end formation of U1 snRNA precursors is coupled to transcription from snRNA promoters.
1986,
Pubmed
