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The nuclear migration signal of Xenopus laevis nucleoplasmin.
Bürglin TR
,
De Robertis EM
.
???displayArticle.abstract??? Nucleoplasmin is the most abundant protein in the nucleus of Xenopus laevis oocytes. Its ability to target to the nucleus when microinjected into the cytoplasm has been the subject of many studies central to our understanding of how proteins segregate into nuclei. Using a cDNA clone we constructed beta-galactosidase-nucleoplasmin hybrids in modified bacterial expression vectors. The fusion proteins were expressed in Escherichia coli, purified and injected into the cytoplasm of X. laevis oocytes. The distribution of the fusion proteins between the cytoplasmic and nuclear compartments were analysed after incubation of various lengths of time. The results show that the signal sequence for nuclear transport is located close to the carboxy terminus of the protein. The signal sequence has been mapped to a small stretch of amino acids, containing a stretch of four lysines analogous to the SV40 large-T antigen signal.
Bradford,
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
1976, Pubmed
Bradford,
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
1976,
Pubmed
Bürglin,
Cloning of nucleoplasmin from Xenopus laevis oocytes and analysis of its developmental expression.
1987,
Pubmed
,
Xenbase
Davey,
Identification of the sequence responsible for the nuclear accumulation of the influenza virus nucleoprotein in Xenopus oocytes.
1985,
Pubmed
,
Xenbase
De Robertis,
Nucleocytoplasmic segregation of proteins and RNAs.
1983,
Pubmed
De Robertis,
Intracellular migration of nuclear proteins in Xenopus oocytes.
1978,
Pubmed
,
Xenbase
De Robertis,
Intracellular transport of microinjected 5S and small nuclear RNAs.
1982,
Pubmed
,
Xenbase
Dingwall,
A polypeptide domain that specifies migration of nucleoplasmin into the nucleus.
1982,
Pubmed
,
Xenbase
Dingwall,
Nucleoplasmin cDNA sequence reveals polyglutamic acid tracts and a cluster of sequences homologous to putative nuclear localization signals.
1987,
Pubmed
,
Xenbase
Earnshaw,
Assembly of nucleosomes: the reaction involving X. laevis nucleoplasmin.
1980,
Pubmed
,
Xenbase
Feldherr,
Evidence for mediated protein uptake by amphibian oocyte nuclei.
1983,
Pubmed
Feldherr,
Movement of a karyophilic protein through the nuclear pores of oocytes.
1984,
Pubmed
,
Xenbase
Feldherr,
Mechanism for the selection of nuclear polypeptides in Xenopus oocytes. II. Two-dimensional gel analysis.
1980,
Pubmed
,
Xenbase
Fischer-Fantuzzi,
Deletion of 43 amino acids in the NH2-terminal half of the large tumor antigen of simian virus 40 results in a non-karyophilic protein capable of transforming established cells.
1985,
Pubmed
Fowler,
Purification, structure, and properties of hybrid beta-galactosidase proteins.
1983,
Pubmed
Goldfarb,
Synthetic peptides as nuclear localization signals.
,
Pubmed
,
Xenbase
Gurdon,
Injected nuclei in frog oocytes: fate, enlargement, and chromatin dispersal.
1976,
Pubmed
,
Xenbase
Hall,
Targeting of E. coli beta-galactosidase to the nucleus in yeast.
1984,
Pubmed
Kalderon,
Sequence requirements for nuclear location of simian virus 40 large-T antigen.
,
Pubmed
Kalderon,
A short amino acid sequence able to specify nuclear location.
1984,
Pubmed
Krippl,
Mapping of functional domains in adenovirus E1A proteins.
1985,
Pubmed
Krohne,
Immunological identification and localization of the predominant nuclear protein of the amphibian oocyte nucleus.
1980,
Pubmed
,
Xenbase
Lanford,
Construction and characterization of an SV40 mutant defective in nuclear transport of T antigen.
1984,
Pubmed
Lanford,
Induction of nuclear transport with a synthetic peptide homologous to the SV40 T antigen transport signal.
1986,
Pubmed
Laskey,
Nucleosomes are assembled by an acidic protein which binds histones and transfers them to DNA.
1978,
Pubmed
,
Xenbase
Messing,
A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments.
1982,
Pubmed
Mills,
An acidic protein which assembles nucleosomes in vitro is the most abundant protein in Xenopus oocyte nuclei.
1980,
Pubmed
,
Xenbase
Moreland,
Identification of a nuclear localization signal of a yeast ribosomal protein.
1985,
Pubmed
Newmeyer,
Assembly in vitro of nuclei active in nuclear protein transport: ATP is required for nucleoplasmin accumulation.
1986,
Pubmed
,
Xenbase
Paine,
Nuclear envelope permeability.
1975,
Pubmed
Richardson,
Nuclear location signals in polyoma virus large-T.
1986,
Pubmed
Richter,
A first exon-encoded domain of E1A sufficient for posttranslational modification, nuclear-localization, and induction of adenovirus E3 promoter expression in Xenopus oocytes.
1985,
Pubmed
,
Xenbase
Rüther,
Easy identification of cDNA clones.
1983,
Pubmed
Sanger,
DNA sequencing with chain-terminating inhibitors.
1977,
Pubmed
Silver,
Amino terminus of the yeast GAL4 gene product is sufficient for nuclear localization.
1984,
Pubmed
Stanley,
Construction of a new family of high efficiency bacterial expression vectors: identification of cDNA clones coding for human liver proteins.
1984,
Pubmed
Towbin,
Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
1979,
Pubmed
Twigg,
Trans-complementable copy-number mutants of plasmid ColE1.
1980,
Pubmed
Ullmann,
One-step purification of hybrid proteins which have beta-galactosidase activity.
1984,
Pubmed
Wychowski,
A domain of SV40 capsid polypeptide VP1 that specifies migration into the cell nucleus.
1986,
Pubmed
Young,
Yeast RNA polymerase II genes: isolation with antibody probes.
1983,
Pubmed
Young,
Efficient isolation of genes by using antibody probes.
1983,
Pubmed
