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Novobiocin interferes with the binding of transcription factors TFIIIA and TFIIIC to the promoters of class III genes.
Van Dyke MW
,
Roeder RG
.
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Novobiocin has been shown to inhibit class III gene transcription from both chromatin and non-chromatin templates. Since novobiocin is a well characterized inhibitor of type II DNA topoisomerases, it has been postulated that a gyrase activity is necessary for transcription. Using DNase I footprinting, we show here that novobiocin inhibits the specific binding of polymerase III transcription factors TFIIIA and TFIIIC to the promoters of the 5S RNA and VA RNA genes, respectively. Concentrations of novobiocin employed were comparable to those necessary to inhibit HeLa topoisomerase II. In vitro transcription assays, performed under equivalent conditions, demonstrated that similar novobiocin concentrations were necessary for transcription inhibition. These results strongly suggest that novobiocin interferes with transcription by inhibiting specific protein-DNA interactions.
Bieker,
Formation of a rate-limiting intermediate in 5S RNA gene transcription.
1985, Pubmed,
Xenbase
Bieker,
Formation of a rate-limiting intermediate in 5S RNA gene transcription.
1985,
Pubmed
,
Xenbase
Bogenhagen,
A control region in the center of the 5S RNA gene directs specific initiation of transcription: II. The 3' border of the region.
1980,
Pubmed
,
Xenbase
Carey,
Analysis of RNA polymerase III transcription complexes by gel filtration.
1986,
Pubmed
,
Xenbase
Cotten,
Novobiocin precipitates histones at concentrations normally used to inhibit eukaryotic type II topoisomerase.
1986,
Pubmed
Dignam,
Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.
1983,
Pubmed
Engelke,
Specific interaction of a purified transcription factor with an internal control region of 5S RNA genes.
1980,
Pubmed
,
Xenbase
Felts,
Novobiocin inhibits interactions required for yeast TFIIIB sequestration during stable transcription complex formation in vitro.
1987,
Pubmed
Gellert,
Novobiocin and coumermycin inhibit DNA supercoiling catalyzed by DNA gyrase.
1976,
Pubmed
Glikin,
In vitro transcription by Xenopus oocytes RNA polymerase III requires a DNA topoisomerase II activity.
1986,
Pubmed
,
Xenbase
Gottesfeld,
Novobiocin inhibits RNA polymerase III transcription in vitro by a mechanism distinct from DNA topoisomerase II.
1986,
Pubmed
Hanas,
Binding of Xenopus transcription factor A to 5S RNA and to single stranded DNA.
1984,
Pubmed
,
Xenbase
Hsieh,
ATP-dependent DNA topoisonmerase from D. melanogaster reversibly catenates duplex DNA rings.
1980,
Pubmed
Kmiec,
Gyration is required for 5S RNA transcription from a chromatin template.
1986,
Pubmed
,
Xenbase
Lassar,
Transcription of class III genes: formation of preinitiation complexes.
1983,
Pubmed
,
Xenbase
Miller,
A homogeneous type II DNA topoisomerase from HeLa cell nuclei.
1981,
Pubmed
Nagata,
Specific binding of a cellular DNA replication protein to the origin of replication of adenovirus DNA.
1983,
Pubmed
Sawadogo,
Interaction of a gene-specific transcription factor with the adenovirus major late promoter upstream of the TATA box region.
1985,
Pubmed
Sealy,
Novobiocin inhibits passive chromatin assembly in vitro.
1986,
Pubmed
,
Xenbase
Segall,
Multiple factors are required for the accurate transcription of purified genes by RNA polymerase III.
1980,
Pubmed
Setzer,
Formation and stability of the 5 S RNA transcription complex.
1985,
Pubmed
,
Xenbase
Shastry,
Multiple factors involved in the transcription of class III genes in Xenopus laevis.
1982,
Pubmed
,
Xenbase
Sugino,
Energy coupling in DNA gyrase and the mechanism of action of novobiocin.
1978,
Pubmed
Van Dyke,
Multiple proteins bind to VA RNA genes of adenovirus type 2.
1987,
Pubmed
