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Genetic complementation in the Xenopus oocyte: co-expression of sea urchin histone and U7 RNAs restores 3' processing of H3 pre-mRNA in the oocyte.
Strub K
,
Birnstiel ML
.
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Efficient 3' processing of sea urchin histone H3 pre-mRNA in the Xenopus oocyte is dependent on co-injection of sea urchin U7 RNA or U7 RNP. We show that U7 RNP is a genuine member of the (Sm-type) U-snRNP family as judged by immunoprecipitation of the U7 RNA and U7 RNPs with polyclonal anti-trimethylguanosine and monoclonal anti-Sm antibodies. Sea urchin U7 RNA can be produced in the frog oocyte from RNA genes driven by a Xenopus U2 promoter. The transcripts thus synthesized have all the known characteristics of authentic U7 RNA. Our experiments further suggest that sequences near the 5' end of the U7 RNA mediate Sm-immunoprecipitability. Co-expression of sea urchin U7 RNA gene and H3 histone gene sequences in the frog oocyte complements faulty 3' maturation of histone pre-mRNA. This provides irrefutable evidence that the sea urchin U7 RNA identified earlier is indeed an essential component of the 3' processing of histone mRNAs.
Altman,
Aspects of biochemical catalysis.
1984, Pubmed
Altman,
Aspects of biochemical catalysis.
1984,
Pubmed
Ares,
Orientation-dependent transcriptional activator upstream of a human U2 snRNA gene.
1985,
Pubmed
,
Xenbase
Benoist,
In vivo sequence requirements of the SV40 early promotor region.
1981,
Pubmed
Berk,
Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids.
1977,
Pubmed
Birnstiel,
Transcription termination and 3' processing: the end is in site!
1985,
Pubmed
Black,
U2 as well as U1 small nuclear ribonucleoproteins are involved in premessenger RNA splicing.
1985,
Pubmed
Brown,
Structure of the sea urchin U1 RNA repeat.
1985,
Pubmed
Cech,
The generality of self-splicing RNA: relationship to nuclear mRNA splicing.
1986,
Pubmed
Chabot,
The 3' splice site of pre-messenger RNA is recognized by a small nuclear ribonucleoprotein.
1985,
Pubmed
Chen,
Yeast mRNA initiation sites are determined primarily by specific sequences, not by the distance from the TATA element.
1985,
Pubmed
De Lorenzi,
Analysis of a sea urchin gene cluster coding for the small nuclear U7 RNA, a rare RNA species implicated in the 3' editing of histone precursor mRNAs.
1986,
Pubmed
De Robertis,
Intracellular transport of microinjected 5S and small nuclear RNAs.
1982,
Pubmed
,
Xenbase
Frederiksen,
The differential inhibitory effect of alpha-amanitin on the synthesis of low molecular weight RNA components in BHK cells.
1978,
Pubmed
Galli,
Biochemical complementation with RNA in the Xenopus oocyte: a small RNA is required for the generation of 3' histone mRNA termini.
1983,
Pubmed
,
Xenbase
Georgiev,
The conserved CAAGAAAGA spacer sequence is an essential element for the formation of 3' termini of the sea urchin H3 histone mRNA by RNA processing.
1985,
Pubmed
Gick,
Generation of histone mRNA 3' ends by endonucleolytic cleavage of the pre-mRNA in a snRNP-dependent in vitro reaction.
1986,
Pubmed
Grosschedl,
Identification of regulatory sequences in the prelude sequences of an H2A histone gene by the study of specific deletion mutants in vivo.
1980,
Pubmed
,
Xenbase
Grosschedl,
Spacer DNA sequences upstream of the T-A-T-A-A-A-T-A sequence are essential for promotion of H2A histone gene transcription in vivo.
1980,
Pubmed
,
Xenbase
Grosveld,
Localization of DNA sequences necessary for transcription of the rabbit beta-globin gene in vitro.
1981,
Pubmed
Grummt,
Ribosomal RNA transcription in vitro is species specific.
1982,
Pubmed
Guerrier-Takada,
The RNA moiety of ribonuclease P is the catalytic subunit of the enzyme.
1983,
Pubmed
Hernandez,
Formation of the 3' end of U1 snRNA is directed by a conserved sequence located downstream of the coding region.
1985,
Pubmed
Krainer,
Multiple factors including the small nuclear ribonucleoproteins U1 and U2 are necessary for pre-mRNA splicing in vitro.
1985,
Pubmed
Kruger,
Self-splicing RNA: autoexcision and autocyclization of the ribosomal RNA intervening sequence of Tetrahymena.
1982,
Pubmed
Krämer,
The 5' terminus of the RNA moiety of U1 small nuclear ribonucleoprotein particles is required for the splicing of messenger RNA precursors.
1984,
Pubmed
Kunkel,
Transcription boundaries of U1 small nuclear RNA.
1985,
Pubmed
Lerner,
Are snRNPs involved in splicing?
1980,
Pubmed
Lerner,
Antibodies to small nuclear RNAs complexed with proteins are produced by patients with systemic lupus erythematosus.
1979,
Pubmed
Lerner,
Monoclonal antibodies to nucleic acid-containing cellular constituents: probes for molecular biology and autoimmune disease.
1981,
Pubmed
,
Xenbase
Mattaj,
Xenopus laevis U2 snRNA genes: tandemly repeated transcription units sharing 5' and 3' flanking homology with other RNA polymerase II transcribed genes.
1983,
Pubmed
,
Xenbase
Mattaj,
Nuclear segregation of U2 snRNA requires binding of specific snRNP proteins.
1985,
Pubmed
,
Xenbase
Mattaj,
An enhancer-like sequence within the Xenopus U2 gene promoter facilitates the formation of stable transcription complexes.
,
Pubmed
,
Xenbase
Melton,
Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.
1984,
Pubmed
Mishima,
Fractionation and reconstitution of factors required for accurate transcription of mammalian ribosomal RNA genes: identification of a species-dependent initiation factor.
1982,
Pubmed
Moore,
Accurate cleavage and polyadenylation of exogenous RNA substrate.
1985,
Pubmed
Nagawa,
The relationship between the "TATA" sequence and transcription initiation sites at the HIS4 gene of Saccharomyces cerevisiae.
1985,
Pubmed
Reddy,
Substitutions, insertions, and deletions in two highly conserved U3 RNA species.
1980,
Pubmed
Reddy,
Small nuclear RNAs and RNA processing.
1983,
Pubmed
Reddy,
Primary and secondary structure of U8 small nuclear RNA.
1985,
Pubmed
Rogers,
A mechanism for RNA splicing.
1980,
Pubmed
Roop,
Structure and expression of a chicken gene coding for U1 RNA.
1981,
Pubmed
Skuzeski,
Synthesis of human U1 RNA. II. Identification of two regions of the promoter essential for transcription initiation at position +1.
1984,
Pubmed
,
Xenbase
Strub,
The cDNA sequences of the sea urchin U7 small nuclear RNA suggest specific contacts between histone mRNA precursor and U7 RNA during RNA processing.
1984,
Pubmed
,
Xenbase
Weaver,
Mapping of RNA by a modification of the Berk-Sharp procedure: the 5' termini of 15 S beta-globin mRNA precursor and mature 10 s beta-globin mRNA have identical map coordinates.
1979,
Pubmed
Westin,
Human U2 and U1 RNA genes use similar transcription signals.
1984,
Pubmed
,
Xenbase
Yuo,
Sequences required for 3' end formation of human U2 small nuclear RNA.
1985,
Pubmed
,
Xenbase
Zinn,
Identification of two distinct regulatory regions adjacent to the human beta-interferon gene.
1983,
Pubmed
