Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
XB-ART-28654
Mol Cell Biol. June 1, 1986; 6 (6): 2053-61.

Efficient homologous recombination of linear DNA substrates after injection into Xenopus laevis oocytes.

Carroll D , Wright SH , Wolff RK , Grzesiuk E , Maryon EB .


Abstract
When DNA molecules are injected into Xenopus oocyte nuclei, they can recombine with each other. With bacteriophage lambda DNAs, it was shown that this recombination is stimulated greatly by introduction of double-strand breaks into the substrates and is dependent on homologous overlaps in the recombination interval. With plasmid DNAs it was shown that little or no recombination occurs between circular molecules but both intra- and intermolecular events take place very efficiently with linear molecules. As with the lambda substrates, homology was required to support recombination; no simple joining of ends was observed. Blockage of DNA ends with nonhomologous sequences interfered with recombination, indicating that ends are used directly to initiate homologous interactions. These observations are combined to evaluate possible models of recombination in the oocytes. Because each oocyte is capable of recombining nanogram quantities of linear DNA, this system offers exceptional opportunities for detailed molecular analysis of the recombination process in a higher organism.

PubMed ID: 2946937
PMC ID: PMC367745
Article link: Mol Cell Biol.
Grant support: GM22232 NIGMS NIH HHS , GM22232 NIGMS NIH HHS



References:
Baker, 1977, Pubmed[+]


My Xenbase: [ Log-in / Register ]
version: [4.5.0]

Major funding for Xenbase is provided by the National Institute of Child Health and Human Development, grant P41 HD064556