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Determination of human factor VIII (AHG-associated protein)--antigen in endothelial cells from Xenopus laevis (XTH cells). Evaluation of a sensitive ELISA technique.
Schlage WK
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AHG-associated protein (AHG-a.p.), the antigen of the blood-clotting factor VIII complex, is a specific endothelial cell marker. Primary (p-XTH) and established (XTH-2) endothelial cells from the hearts of Xenopus laevis tadpoles were assayed for the presence of this marker by means of immunological cross-reaction (recognition of common antigenic sites) with antiserum against human AHG-a.p. Radial immunodiffusion and rocket immunoelectrophoresis proved to be insufficiently sensitive, whereas immunofluorescence and a newly evaluated ELISA technique gave positive results. The very high sensitivity of the ELISA (less than 1/240,000 of the AHG-a.p. in 0.1 ml human standard plasma can be detected) and the removal of interfering proteins by gel filtration also revealed the presence of AHG-a.p. in the fetal calf serum used in the culture medium; earlier investigations into this subject by a one-step radioimmunoassay had reported negative results. Specially adapted XTH-2 cells were grown in a protein-and serum-free hydrolysate medium in order to demonstrate the presence of a Xenopus-derived antigen that was immunoreactive with the anti-human AHG-a.p.
Bereiter-Hahn,
Interaction of metabolic inhibitors with actin fibrils.
1984, Pubmed,
Xenbase
Bereiter-Hahn,
Interaction of metabolic inhibitors with actin fibrils.
1984,
Pubmed
,
Xenbase
Bereiter-Hahn,
Dimethylaminostyrylmethylpyridiniumiodine (daspmi) as a fluorescent probe for mitochondria in situ.
1976,
Pubmed
Bradford,
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
1976,
Pubmed
Del Vecchio,
Expression of angiotensin-converting enzyme activity in cultured pulmonary artery endothelial cells.
1981,
Pubmed
Del Vecchio,
Aging of endothelium in culture: decrease in angiotensin-converting enzyme activity.
1982,
Pubmed
Engvall,
Enzyme immunoassay ELISA and EMIT.
1980,
Pubmed
Gospodarowicz,
Factors involved in the modulation of cell proliferation in vivo and in vitro: the role of fibroblast and epidermal growth factors in the proliferative response of mammalian cells.
1978,
Pubmed
Hayman,
Distribution of fetal bovine serum fibronectin and endogenous rat cell fibronectin in extracellular matrix.
1979,
Pubmed
Hormia,
Expression of factor VIII-related antigen and Ulex lectin binding sites in endothelial cells during long-term culture.
1982,
Pubmed
Hoyer,
Immunologic studies of antihemophilic factor (AHF, factor VIII). IV. Radioimmunoassay of AHF antigen.
1972,
Pubmed
Jaffe,
Synthesis of antihemophilic factor antigen by cultured human endothelial cells.
1973,
Pubmed
Jaffe,
Culture of human endothelial cells derived from umbilical veins. Identification by morphologic and immunologic criteria.
1973,
Pubmed
Jaye,
Modulation of the sis gene transcript during endothelial cell differentiation in vitro.
1985,
Pubmed
Kunzenbacher,
Dynamics of the cytoskeleton of epidermal cells in situ and in culture.
1982,
Pubmed
,
Xenbase
Laurell,
Quantitative estimation of proteins by electrophoresis in agarose gel containing antibodies.
1966,
Pubmed
Maciag,
Organizational behavior of human umbilical vein endothelial cells.
1982,
Pubmed
Marusyk,
A simple method for dialysis of small-volume samples.
1980,
Pubmed
NEUMAN,
Serumless medium for cultivation of cells of normal and malignant origin.
1960,
Pubmed
Porstmann,
Comparison of chromogens for the determination of horseradish peroxidase as a marker in enzyme immunoassay.
1981,
Pubmed
Rose,
The HFH-18 mouse melanoma in roller tube, chamber, and circumfusion system cultures.
1969,
Pubmed
Rosen,
Proliferative characteristics of clonal endothelial cell strains.
1981,
Pubmed
Ryan,
Endothelial cells of bovine pulmonary artery lack receptors for C3b and for the Fc portion of immunoglobulin G.
1980,
Pubmed
Ryan,
Isolation and culture of pulmonary artery endothelial cells.
1978,
Pubmed
Santoro,
Preferential binding of high molecular weight forms of von Willebrand factor to fibrillar collagen.
1983,
Pubmed
Schlage,
A microscope perfusion respirometer for continuous respiration measurement of cultured cells during microscopic observation.
1983,
Pubmed
,
Xenbase
Schwartz,
Selection and characterization of bovine aortic endothelial cells.
1978,
Pubmed
WOLF,
AMPHIBIAN CELL CULTURE: PERMANENT CELL LINE FROM THE BULLFROG (RANA CATESBEIANA).
1964,
Pubmed
