XB-ART-29128EMBO J August 1, 1985; 4 (8): 1927-31.
Mediation of anion transport in oocytes of Xenopus laevis by biosynthetically inserted band 3 protein from mouse spleen erythroid cells.
mRNA from the spleens of anemic mice was purified by oligo(dT)-cellulose chromatography and fractionated by density gradient centrifugation. After injection into oocytes of Xenopus laevis, two of the four fractions obtained led, after 16 h of incubation at 20 degrees C, to the expression of mouse band 3 protein, as demonstrated by immunoprecipitation with polyclonal antibodies against mouse band 3. Flux measurements showed an approximately 2- to 4-fold increment of 36Cl- uptake, which could be abolished by two different stilbene disulfonates, specific inhibitors of band 3-mediated anion transport in red blood cells.
PubMed ID: 2415354
PMC ID: PMC554442
Species referenced: Xenopus
Genes referenced: slc4a1
References [+] :
Anderson, Immunoprecipitation of proteins from cell-free translations. 1984, Pubmed