XB-ART-29128
EMBO J
August 1, 1985;
4
(8):
1927-31.
Mediation of anion transport in oocytes of Xenopus laevis by biosynthetically inserted band 3 protein from mouse spleen erythroid cells.
Morgan M
,
Hanke P
,
Grygorczyk R
,
Tintschl A
,
Fasold H
,
Passow H
.
Abstract
mRNA from the spleens of anemic mice was purified by oligo(
dT)-cellulose chromatography and fractionated by density gradient centrifugation. After injection into oocytes of Xenopus laevis, two of the four fractions obtained led, after 16 h of incubation at 20 degrees C, to the expression of mouse
band 3 protein, as demonstrated by immunoprecipitation with polyclonal antibodies against mouse
band 3. Flux measurements showed an approximately 2- to 4-fold increment of 36Cl- uptake, which could be abolished by two different stilbene disulfonates, specific inhibitors of
band 3-mediated anion transport in red
blood cells.
PubMed ID:
2415354
PMC ID:
PMC554442
Article link:
Species referenced:
Xenopus
Genes referenced:
slc4a1
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