Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
EMBO J
1985 Mar 01;43:823-7. doi: 10.1002/j.1460-2075.1985.tb03704.x.
Show Gene links
Show Anatomy links
Queuosine modification of the wobble base in tRNAHis influences 'in vivo' decoding properties.
Meier F
,
Suter B
,
Grosjean H
,
Keith G
,
Kubli E
.
???displayArticle.abstract???
The 'in vivo' decoding properties of four tRNAHis isoacceptors, two from Drosophila melanogaster and two from brewer's yeast, were studied after their microinjection, along with turnip yellow mosaic virus (TYMV) coat protein mRNA, into Xenopus laevis oocytes. The two Drosophila isoacceptors are identical besides containing either a guanosine (G) or the hypermodified nucleoside queuosine (Q) in the wobble position. The brewer's yeast isoacceptors differ by four bases in the anticodon stem, and by one base in the amino acceptor stem. Our results show that, under competing 'in vivo' conditions, the Drosophila tRNAHis with the anticodon GUG clearly prefers the histidine codon CAC to the codon CAU, whereas little preference is observed for the tRNAHis with the anticodon QUG for the codon CAU, and no preference for either codon by the two yeast isoacceptors. Hence, it can be concluded that the presence of the Q-base clearly affects the choice of the codon. This is the first demonstration of an 'in vivo' codon preference by tRNA isoacceptors differing in the modification of the wobble base during the elongation step of protein synthesis. These results imply that one function of the Q-base is at the translational level.
Altwegg,
The nucleotide sequence of histidine tRNA gamma of Drosophila melanogaster.
1980, Pubmed
Altwegg,
The nucleotide sequence of histidine tRNA gamma of Drosophila melanogaster.
1980,
Pubmed
Bienz,
Nonsense suppression in eukaryotes: the use of the Xenopus oocyte as an in vivo assay system.
1980,
Pubmed
,
Xenbase
Carter,
Scintillation counting: a comparison of the counting efficiencies of serveral aqueous solubilizers.
1973,
Pubmed
Clarkson,
Reiterated transfer RNA genes of Xenopus laevis.
1973,
Pubmed
,
Xenbase
Gouy,
Codon usage in bacteria: correlation with gene expressivity.
1982,
Pubmed
Grigliatti,
Studies on the transfer RNA genes of Drosophila.
1974,
Pubmed
Grosjean,
On the physical basis for ambiguity in genetic coding interactions.
1978,
Pubmed
Grosjean,
Preferential codon usage in prokaryotic genes: the optimal codon-anticodon interaction energy and the selective codon usage in efficiently expressed genes.
1982,
Pubmed
Guilley,
Nucleotide sequence of turnip yellow mosaic virus coat protein mRNA.
1978,
Pubmed
Hansen,
Reversible inhibition by histidinol of protein synthesis in human cells at the activation of histidine.
1972,
Pubmed
Hatfield,
The aminoacyl-tRNA population of human reticulocytes.
1982,
Pubmed
Hosbach,
Transfer RNA in aging Drosophila: II. Isoacceptor patterns.
1979,
Pubmed
Kasai,
Distribution of the modified nucleoside Q and its derivatives in animal and plant transfer RNA's.
1975,
Pubmed
Keith,
Primary structure of three tRNAs from brewer's yeast: tRNAPro2, tRNAHis1 and tRNAHis2.
1983,
Pubmed
Keith,
[Optimal conditions of aminoacylation of yeast tRNA Asp and tRNA Trp].
1971,
Pubmed
Kubli,
The localization of tRNA4Glu genes from Drosophila melanogaster by "in situ" hybridization.
1978,
Pubmed
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Murgola,
Codon context effects in missense suppression.
1984,
Pubmed
Nishimura,
Structure, biosynthesis, and function of queuosine in transfer RNA.
1983,
Pubmed
Noguchi,
Isolation and characterization of an Escherichia coli mutant lacking tRNA-guanine transglycosylase. Function and biosynthesis of queuosine in tRNA.
1982,
Pubmed
Olsen,
Chromatographic and functional comparison of human placenta and HeLa cell tyrosine transfer ribonucleic acids.
1976,
Pubmed
Smith,
The effect of the Q base modification on the usage of tRNAHis in globin synthesis.
1982,
Pubmed
Sprinzl,
Compilation of tRNA sequences.
1984,
Pubmed
White,
Activity of a transfer RNA modifying enzyme during the development of Drosophila and its relationship to the su(s) locus.
1973,
Pubmed
Yarus,
Translational efficiency of transfer RNA's: uses of an extended anticodon.
1982,
Pubmed
