XB-ART-29685Nucleic Acids Res June 25, 1984; 12 (12): 4921-38.
Characterization of highly and moderately repetitive 500 bp Eco RI fragments from Xenopus laevis DNA.
Three different types of repetitive Eco RI fragments, which comigrate within a visible band of approximately 500 bp at gel electrophoresis of Xenopus laevis DNA Eco RI digests have been cloned and sequenced. These sequences are designated as Repetitive Eco RI Monomers: REM 1, REM 2 and REM 3. The sequences contain direct repeats, inverted repeats and palindromic elements. Genomic organization of the most abundant sequence (REM 1; 0.4% of total DNA) is that of an interspersed sequence. REM 2 (0.08%) is partly organized as an interspersed element and partly found in tandem arrangement, whereas REM 3 (0.02%) represents the tandemly repeated monomeric unit of a satellite DNA. In situ hybridization has shown that REM 1 and REM 2 sequences are found on most chromosomes, REM 1 being preferentially located on specific chromosomal loci. REM 3 is located near the centromere region of only one chromosome pair (presumably number 1). Hybridization of Northern blots from RNAs of different developmental stages revealed that REM 1, REM 2 and REM 3 sequences are transcribed and that transcription is under developmental control.
PubMed ID: 6330690
PMC ID: PMC318889
Article link: Nucleic Acids Res
Genes referenced: rem1
References [+] :
Ackerman, Molecular cloning and sequencing of OAX DNA: an abundant gene family transcribed and activated in Xenopus oocytes. 2000, Pubmed, Xenbase