XB-ART-29827Nature February 16, 1984; 307 (5952): 604-8.
Expression of functional acetylcholine receptor from cloned cDNAs.
The cloned cDNAs encoding the four subunits of the Torpedo californica acetylcholine receptor, each carried by a simian virus 40 vector, direct the synthesis of the functional receptor in a combined expression system consisting of COS monkey cells and Xenopus oocytes. Our results suggest that all four subunits are required to elicit a normal nicotinic response to acetylcholine, whereas only the alpha-subunit is indispensable for alpha-bungarotoxin binding activity.
PubMed ID: 6320016