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Cell February 1, 1983; 32 (2): 425-34.

Nucleocytoplasmic distribution of snRNPs and stockpiled snRNA-binding proteins during oogenesis and early development in Xenopus laevis.

Zeller R , Nyffenegger T , De Robertis EM .

The distribution of small nuclear ribonucleoprotein particles containing U snRNAs (U snRNPs) during oogenesis and early development in Xenopus was analyzed with a lupus antibody (anti-Sm) that reacts with snRNA-binding proteins. Fully grown oocytes and embryos prior to gastrulation were found to be relatively depleted of U snRNPs in their nuclei and to contain an excess of snRNA-binding proteins stored in the cytoplasm. During late blastula-early gastrula, or after microinjection of U snRNAs into the cytoplasm of a mature oocyte, the proteins migrate into the nucleus. Dot hybridization analysis showed that small previtellogenic oocytes already contain a maximal amount of U1 (and U2) snRNAs, which then decreases to about 20% of that value in fully mature oocytes, even though the cell''s volume has increased enormously. Thus fully grown oocytes and eggs accumulate snRNA-binding proteins for use during early development, but this is not coupled with the accumulation of U snRNA.

PubMed ID: 6186395
Article link: Cell