Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
EMBO J
1983 Jan 01;28:1417-22. doi: 10.1002/j.1460-2075.1983.tb01600.x.
Show Gene links
Show Anatomy links
Molecular cloning and sequencing of OAX DNA: an abundant gene family transcribed and activated in Xenopus oocytes.
Ackerman EJ
.
???displayArticle.abstract???
OAX DNA codes for a 181 nucleotide long RNA whose transcription is strongly activated in somatic nuclei after their injection into a Xenopus oocytenucleus. OAX RNA can be transcribed in vitro using an extract of Xenopus oocyte nuclei and total genomic DNA. Hybridization with OAX RNA as a probe indicates that OAX DNA is abundant in the Xenopus genome (at least 10(4) copies per genome). OAX DNA is present in tandemly repeated HindIII units of 752 bp. The complete DNA sequence of one of these OAX HindIII units is reported here. The OAX RNA transcript has been mapped within the OAX HindIII unit using S1 nuclease. Microinjection into Xenopus oocyte nuclei of either the OAX HindIII unit or a subclone containing only the RNA coding portion of the OAX HindIII unit both produce OAX RNA transcripts. This shows that the OAX promoter lies within the coding region of the RNA. The OAX RNA sequence has two elements which fit the RNA polymerase III promoter consensus sequence, and shows homology with dispersed RNA polymerase III transcription units in mammals.
Anderson,
Sequence organization of the poly(A) RNA synthesized and accumulated in lampbrush chromosome stage Xenopus laevis oocytes.
1982, Pubmed,
Xenbase
Anderson,
Sequence organization of the poly(A) RNA synthesized and accumulated in lampbrush chromosome stage Xenopus laevis oocytes.
1982,
Pubmed
,
Xenbase
Barta,
Primary structure and evolution of rat growth hormone gene.
1981,
Pubmed
Berk,
Spliced early mRNAs of simian virus 40.
1978,
Pubmed
Birkenmeier,
A nuclear extract of Xenopus laevis oocytes that accurately transcribes 5S RNA genes.
1978,
Pubmed
,
Xenbase
Bogenhagen,
Nucleotide sequences in Xenopus 5S DNA required for transcription termination.
1981,
Pubmed
,
Xenbase
Chambers,
Dissection of a replication origin of Xenopus DNA.
1982,
Pubmed
,
Xenbase
Ciliberto,
Common and interchangeable elements in the promoters of genes transcribed by RNA polymerase iii.
1983,
Pubmed
,
Xenbase
Davidson,
Repetitive sequence transcripts in development.
1982,
Pubmed
Galli,
Two conserved sequence blocks within eukaryotic tRNA genes are major promoter elements.
1981,
Pubmed
,
Xenbase
Gurdon,
Methods for nuclear transplantation in amphibia.
1977,
Pubmed
Heidecker,
A versatile primer for DNA sequencing in the M13mp2 cloning system.
1980,
Pubmed
Jelinek,
Repetitive sequences in eukaryotic DNA and their expression.
1982,
Pubmed
Krayev,
Ubiquitous transposon-like repeats B1 and B2 of the mouse genome: B2 sequencing.
1982,
Pubmed
Kressmann,
A tRNA gene of Xenopus laevis contains at least two sites promoting transcription.
1979,
Pubmed
,
Xenbase
Lemischka,
The sequences of an expressed rat alpha-tubulin gene and a pseudogene with an inserted repetitive element.
1982,
Pubmed
Lerner,
Antibodies to small nuclear RNAs complexed with proteins are produced by patients with systemic lupus erythematosus.
1979,
Pubmed
Messing,
A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments.
1982,
Pubmed
Müller,
Nucleotide sequence of genes coding for tRNAPhe and tRNATyr from a repeating unit of X. laevis DNA.
1980,
Pubmed
,
Xenbase
Patient,
Internal organization of the major adult alpha- and beta-globin genes of X. laevis.
1980,
Pubmed
,
Xenbase
Sanger,
Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing.
1980,
Pubmed
Sanger,
DNA sequencing with chain-terminating inhibitors.
1977,
Pubmed
Sanger,
The use of thin acrylamide gels for DNA sequencing.
1978,
Pubmed
Singer,
SINEs and LINEs: highly repeated short and long interspersed sequences in mammalian genomes.
1982,
Pubmed
Southern,
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
1975,
Pubmed
Spohr,
Organization and sequence analysis of a cluster of repetitive DNA elements from Xenopus laevis.
1981,
Pubmed
,
Xenbase
Spohr,
Structural analysis of a cDNA clone from Xenopus laevis containing a repetitive sequence element.
1982,
Pubmed
,
Xenbase
Staden,
An interactive graphics program for comparing and aligning nucleic acid and amino acid sequences.
1982,
Pubmed
Staden,
Automation of the computer handling of gel reading data produced by the shotgun method of DNA sequencing.
1982,
Pubmed
Staden,
Further procedures for sequence analysis by computer.
1978,
Pubmed
Sutcliffe,
Common 82-nucleotide sequence unique to brain RNA.
1982,
Pubmed
Traboni,
A novel method for site-directed mutagenesis: its application to an eukaryotic tRNAPro gene promoter.
1982,
Pubmed
,
Xenbase
Ullu,
Human 7SL RNA consists of a 140 nucleotide middle-repetitive sequence inserted in an alu sequence.
1982,
Pubmed
Wakefield,
The activation of RNA synthesis by somatic nuclei injected into amphibian oocytes.
1983,
Pubmed
,
Xenbase
