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Immunological localization of a major karyoskeletal protein in nucleoli of oocytes and somatic cells of Xenopus laevis.
Krohne G
,
Stick R
,
Kleinschmidt JA
,
Moll R
,
Franke WW
,
Hausen P
.
???displayArticle.abstract??? Oocyte nuclei of Xenopus laevis contain two major karyoskeletal proteins characterized by their resistance to extractions in high salt buffers and the detergent Triton X-100, i.e. a polypeptide of 68,000 mol wt which is located in the core complex-lamina structure and a polypeptide of 145,000 mol wt enriched in nucleolar fractions. Both proteins are also different by tryptic peptide maps and immunological determinants. Mouse antibodies were raised against insoluble karyoskeletal proteins from Xenopus oocytes and analyzed by immunoblotting procedures. Affinity purified antibodies were prepared using antigens bound to nitrocellulose paper. In immunofluorescence microscopy of Xenopus oocytes purified antibodies against the polypeptide of 145,000 mol wt showed strong staining of nucleoli, with higher concentration in the nucleolar cortex, and of smaller nucleoplasmic bodies. In various other cells including hepatocytes, Sertoli cells, spermatogonia, and cultured kidney epithelial cells antibody staining was localized in small subnucleolar granules. The results support the conclusion that this "insoluble" protein is a major nucleus-specific protein which is specifically associated with--and characteristic of--nucleoli and certain nucleolus-related nuclear bodies. It represents the first case of a positive localization of a karyoskeletal protein in the nuclear interior, i.e. away from the pore complex-lamina structure of the nuclear cortex.
Adolph,
Organization of chromosomes in HeLa cells: isolation of histone-depleted nuclei and nuclear scaffolds.
1980, Pubmed
Adolph,
Organization of chromosomes in HeLa cells: isolation of histone-depleted nuclei and nuclear scaffolds.
1980,
Pubmed
Agutter,
Nuclear non-chromatin proteinaceous structures: their role in the organization and function of the interphase nucleus.
1980,
Pubmed
Bona,
Antibodies of RNA polymerase II (B) inhibit transcription in Lampbrush chromosomes after microinjection into living amphibian oocytes.
1981,
Pubmed
,
Xenbase
Catino,
Subcellular localization of DNA-binding protein BA by immunofluorescence and immunoelectron microscopy.
1979,
Pubmed
Chooi,
An electron microscopic method for localization of ribosomal proteins during transcription of ribosomal DNA: a method for studying protein assembly.
1981,
Pubmed
Davis,
Nucleolar antigen found in several human tumors but not in the nontumor tissues studied.
1979,
Pubmed
Elder,
Radioiodination of proteins in single polyacrylamide gel slices. Tryptic peptide analysis of all the major members of complex multicomponent systems using microgram quantities of total protein.
1977,
Pubmed
Franke,
Organization of transcribed and nontranscribed chromatin.
1980,
Pubmed
Franke,
A nucleolar skeleton of protein filaments demonstrated in amplified nucleoli of Xenopus laevis.
1981,
Pubmed
,
Xenbase
Franke,
The nuclear envelope and the architecture of the nuclear periphery.
1981,
Pubmed
Gerace,
Immunocytochemical localization of the major polypeptides of the nuclear pore complex-lamina fraction. Interphase and mitotic distribution.
1978,
Pubmed
Jamrich,
Functional organization of polytene chromosomes.
1978,
Pubmed
Johnson,
A simple method of reducing the fading of immunofluorescence during microscopy.
1981,
Pubmed
Krohne,
The major polypeptides of the nuclear pore complex.
1978,
Pubmed
,
Xenbase
Krohne,
Localization of a nuclear envelope-associated protein by indirect immunofluorescence microscopy using antibodies against a major polypeptide from rat liver fractions enriched in nuclear envelope-associated material.
1978,
Pubmed
Krohne,
Cell type-specific differences in protein composition of nuclear pore complex-lamina structures in oocytes and erythrocytes of Xenopus laevis.
1981,
Pubmed
,
Xenbase
Lerner,
Monoclonal antibodies to nucleic acid-containing cellular constituents: probes for molecular biology and autoimmune disease.
1981,
Pubmed
,
Xenbase
Miller,
The relationship of ribosomal RNA synthesis to the formation of segregated nucleoli and nucleolus-like bodies.
1976,
Pubmed
,
Xenbase
Moreno Diaz de la Espina,
Medusoid fibril bodies: a novel type of nuclear filament of diameter 8 to 12 nm with periodic ultrastructure demonstrated in oocytes of Xenopus laevis.
1982,
Pubmed
,
Xenbase
Olmsted,
Affinity purification of antibodies from diazotized paper blots of heterogeneous protein samples.
1981,
Pubmed
Phillips,
Nucleolus-like bodies in micronuclei of cultured Xenopus cells.
1979,
Pubmed
,
Xenbase
Scalenghe,
Large scale isolation of nuclei and nucleoli from vitellogenic oocytes of Xenopus laevis.
1978,
Pubmed
,
Xenbase
Scheer,
Effects of actinomycin D on the association of newly formed ribonucleoproteins with the cistrons of ribosomal RNA in Triturus oocytes.
1975,
Pubmed
Scheer,
Experimental disintegration of the nuclear envelope. Evidence for pore-connecting fibrils.
1976,
Pubmed
,
Xenbase
Scheer,
Regulation of transcription of genes of ribosomal rna during amphibian oogenesis. A biochemical and morphological study.
1976,
Pubmed
Stick,
Immunological analysis of nuclear lamina proteins.
1980,
Pubmed
,
Xenbase
Stick,
Immunological localization of the major architectural protein associated with the nuclear envelope of the Xenopus laevis oocyte.
1982,
Pubmed
,
Xenbase
Todorov,
A precursor to the small ribosome in nucleoli of Friend erythroleukemia cells.
1981,
Pubmed
Towbin,
Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
1979,
Pubmed
