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Observations on intracellular pH during cleavage of eggs of Xenopus laevis.
Lee SC
,
Steinhardt RA
.
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Direct measurements of intracellular pH was made with recessed-tip pH microelectrodes in fertilized eggs of the frog, Xenopus laevis, from approximately 1 h after fertilization to mid-blastula. The intracellular pH just before first cleavage was 7.65 +/- 0.04 (SD; n = 9). By stage 5 to the middle of stage 6, average intracellular pH was 7.70 +/- 0.06 (SD; n = 16). A statistically significant alkalization of 0.18 +/- 0.03 pH unit (SD; n = 5) was observed beginning in early blastula. A cycle of less than or equal to 0.05 pH unit was occasionally observed during the pre-blastula period, but its significance is unknown. By exposing the early cleavageembryo to saline buffered with sodium propionate, pH 4.7-5.0, it was possible to lower intracellular pH with some degree of control. Apparently, normal cleavage continued to occur when intracellular pH had been forced as much as 0.3 unit below normal. We conclude that this implies no specific involvement of intracellular pH in mitosis and cytokinesis. If intracellular pH was lowered further, cell division ceased at about pH 7.2, and furrow regression began at about pH 7.0. Once furrow regression occurred, subsequent development was usually arrested or abnormal when the embryo was transferred back to normal saline.
Baker,
Intracellular calcium and cell cleavage in early embryos of Xenopus laevis.
1972, Pubmed,
Xenbase
Baker,
Intracellular calcium and cell cleavage in early embryos of Xenopus laevis.
1972,
Pubmed
,
Xenbase
Bozhkova,
Measurement of electrical coupling between cells of axolotl embryos during cleavage divisions.
1974,
Pubmed
Cande,
A permeabilized cell model for studying cytokinesis using mammalian tissue culture cells.
1980,
Pubmed
Gillies,
Intracellular pH changes during the cell cycle in Tetrahymena.
1979,
Pubmed
Grainger,
Intracellular pH controls protein synthesis rate in the sea urchine egg and early embryo.
1979,
Pubmed
Hollinger,
"Cleavage" and cortical granule breakdown in Rana pipiens oocytes induced by direct microinjection of calcium.
1976,
Pubmed
Ito,
Electrical characteristics of Triturus egg cells during cleavage.
1966,
Pubmed
Lee,
pH changes associated with meiotic maturation in oocytes of Xenopus laevis.
1981,
Pubmed
,
Xenbase
Palmer,
Some bio-electric parameters of early Xenopus embryos.
1970,
Pubmed
,
Xenbase
Ridgway,
Free calcium increases explosively in activating medaka eggs.
1977,
Pubmed
Rink,
Free calcium in Xenopus embryos measured with ion-selective microelectrodes.
1980,
Pubmed
,
Xenbase
Schroeder,
Ionophore A23187, calcium and contractility in frog eggs.
1974,
Pubmed
Schroeder,
Dynamics of the contractile ring.
1975,
Pubmed
Shen,
Direct measurement of intracellular pH during metabolic derepression of the sea urchin egg.
1978,
Pubmed
Shih,
Kinetic analysis of amino acid pools and protein synthesis in amphibian oocytes and embryos.
1978,
Pubmed
,
Xenbase
Slack,
Absence of intracellular potential gradients in amphibian embryos.
1972,
Pubmed
,
Xenbase
Slack,
The distribution of sodium and potassium in amphibian embryos during early development.
1973,
Pubmed
,
Xenbase
Slack,
Intracellular and intercellular potentials in the early amphibian embryo.
1973,
Pubmed
,
Xenbase
Smith,
Uterine suppression of biochemical and morphogenetic events in Rana pipiens.
1970,
Pubmed
Spray,
Gap junctional conductance is a simple and sensitive function of intracellular pH.
1981,
Pubmed
Thoman,
Absence of dorsal-ventral differences in energy metabolism in early embryos of Xenopus laevis.
1979,
Pubmed
,
Xenbase
Thomas,
Intracellular pH of snail neurones measured with a new pH-sensitive glass mirco-electrode.
1974,
Pubmed
Timourian,
Cleavage furrow: calcium as determinant of site.
1972,
Pubmed
Turin,
Intracellular pH in early Xenopus embryos: its effect on current flow between blastomeres.
1980,
Pubmed
,
Xenbase
Webb,
Direct measurement of intracellular pH changes in Xenopus eggs at fertilization and cleavage.
1981,
Pubmed
,
Xenbase
Woodland,
Changes in the polysome content of developing Xenopus laevis embryos.
1974,
Pubmed
,
Xenbase
Woodward,
Electrical signs of new membrane production during cleavage of Rana pipiens eggs.
1968,
Pubmed
de Laat,
Intracellular ionic distribution, cell membrane permeability and membrane potential of the Xenopus egg during first cleavage.
1974,
Pubmed
,
Xenbase
