Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Yeast tRNA3Leu gene transcribed and spliced in a HeLa cell extract.
Standring DN
,
Venegas A
,
Rutter WJ
.
???displayArticle.abstract???
A cloned yeast tRNA3Leu gene containing a 33-base intervening sequence (IVS) is selectively transcribed by a soluble extract from HeLa cells. The 130-nucleotide tRNA3Leu precursor RNA formed is colinear with the gene and contains approximately 4 leader nucleotides and up to 9 trailer nucleotides. The IVS is accurately and efficiently removed by an endogenous HeLa excision-ligase activity to yield the spliced tRNA, the free IVS, and the half-tRNA intermediates. The splicing reaction occurs without prior 5' and 3' maturation of the precursor but, with this exception, this pattern of synthesis and subsequent maturation of the tRNA3Leu precursor conforms to the scheme for tRNA biosynthesis deduced for the xenopus system. Indeed, the two systems utilize similar or identical tRNA3Leu precursors. Our results stress the extraordinary conservation of tRNA biosynthesis in eukaryotes and demonstrate that a HeLa extract provides a useful system for investigating this process.
Abelson,
RNA processing and the intervening sequence problem.
1979, Pubmed
Abelson,
RNA processing and the intervening sequence problem.
1979,
Pubmed
De Robertis,
Transcription and processing of cloned yeast tyrosine tRNA genes microinjected into frog oocytes.
1979,
Pubmed
,
Xenbase
Fowlkes,
Transcriptional control regions of the adenovirus VAI RNA gene.
1980,
Pubmed
Garber,
Transcription of a cloned Bombyx mori tRNA2Ala gene: nucleotide sequence of the tRNA precursor and its processing in vitro.
1979,
Pubmed
Goodman,
Nucleotide sequence of a mutant eukaryotic gene: the yeast tyrosine-inserting ochre suppressor SUP4-o.
1977,
Pubmed
Hopper,
tRNA synthesis: identification of in vivo precursor tRNAs from parental and mutant yeast strains.
1981,
Pubmed
,
Xenbase
Hopper,
A yeast mutant which accumulates precursor tRNAs.
1978,
Pubmed
Johnson,
Transcription and processing of a yeast tRNA gene containing a modified intervening sequence.
1980,
Pubmed
,
Xenbase
Knapp,
Transcription and processing of intervening sequences in yeast tRNA genes.
1978,
Pubmed
Koski,
Mutations of the yeast SUP4 tRNATyr locus: transcription of the mutant genes in vitro.
1980,
Pubmed
,
Xenbase
Kressmann,
A tRNA gene of Xenopus laevis contains at least two sites promoting transcription.
1979,
Pubmed
,
Xenbase
Kressmann,
Transcription of cloned tRNA gene fragments and subfragments injected into the oocyte nucleus of Xenopus laevis.
1978,
Pubmed
,
Xenbase
Kurjan,
Mutations at the yeast SUP4 tRNATyr locus: DNA sequence changes in mutants lacking suppressor activity.
1980,
Pubmed
Maxam,
A new method for sequencing DNA.
1977,
Pubmed
Melton,
Order and intracellular location of the events involved in the maturation of a spliced tRNA.
1980,
Pubmed
,
Xenbase
Müller,
Nucleotide sequence of genes coding for tRNAPhe and tRNATyr from a repeating unit of X. laevis DNA.
1980,
Pubmed
,
Xenbase
O'Farrell,
Structure and processing of yeast precursor tRNAs containing intervening sequences.
1978,
Pubmed
Peebles,
Splicing of yeast tRNA precursors: a two-stage reaction.
1979,
Pubmed
Robinson,
Analysis of a drosophila tRNA gene cluster: two tRNALeu genes contain intervening sequences.
1981,
Pubmed
Selker,
A phenylalanine tRNA gene from Neurospora crassa: conservation of secondary structure involving an intervening sequence.
1980,
Pubmed
Silverman,
The nucleotide sequence of a cloned Drosophila arginine tRNA gene and its in vitro transcription in Xenopus germinal vesicle extracts.
1979,
Pubmed
,
Xenbase
Valenzuela,
Molecular structure of yeast RNA polymerase III: demonstration of the tripartite transcriptive system in lower eukaryotes.
1976,
Pubmed
Valenzuela,
Structure of yeast phenylalanine-tRNA genes: an intervening DNA segment within the region coding for the tRNA.
1978,
Pubmed
Venegas,
Isolation of yeast tRNALeu genes. DNA sequence of a cloned tRNALeu3 gene.
1979,
Pubmed
Wallace,
Directed deletion of a yeast transfer RNA intervening sequence.
1980,
Pubmed
Weil,
Faithful transcription of eukaryotic genes by RNA polymerase III in systems reconstituted with purified DNA templates.
1979,
Pubmed
,
Xenbase
Wu,
Adenovirus DNA-directed transcription of 5.5S RNA in vitro.
1978,
Pubmed
