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The organization of the histone genes in the genome of Xenopus laevis.
van Dongen W
,
de Laaf L
,
Zaal R
,
Moorman A
,
Destrée O
.
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We have studied the organization of the histone genes in the DNA from several individuals of Xenopus laevis. For that purpose, Southern blots of genomic DNA, that was digested with several restriction enzymes, were hybridized with radioactively labeled DNA fragments from clone X1-hi-1 (14), containing genes for Xenopus histones H2A, H2B, H3 and H4. In the DNA of all animals that were screened we found a major repeating unit of 14 kilobasepairs, which contains genes for histones H2A, H2B, H3 and H4 (H1 not tested) and is represented up to 30 times in the genome. The order of the genes in this major repeating unit is H4 - H3 - H2A - H2B. This order is different from that in the histone DNA of clone X1-hi-1, i.e. H3 - H4 - H2A - H2B. In addition to the genes in the major repeating unit, histone genes are present in unique restriction fragments in numbers that vary from one animal to another. The restriction patterns for the histone genes in these unique fragments were found to be different for all eight Xenopus individuals that were screened. The cloned Xenopus histone gene fragment X1-hi-1 represents such a unique fragment and is not present in the DNA of each single individual. The total number of genes coding for each of the nucleosomal histones is 45-50 per haploid genome.
Busslinger,
Ubiquitous and gene-specific regulatory 5' sequences in a sea urchin histone DNA clone coding for histone protein variants.
1980, Pubmed
Busslinger,
Ubiquitous and gene-specific regulatory 5' sequences in a sea urchin histone DNA clone coding for histone protein variants.
1980,
Pubmed
Clarkson,
Integration of eukaryotic genes for 5S RNA and histone proteins into a phage lambda receptor.
1976,
Pubmed
,
Xenbase
Cohn,
Nonallelic histone gene clusters of individual sea urchins (Lytechinus pictus): polarity and gene organization.
1979,
Pubmed
Crawford,
Histone genes are clustered with a 15-kilobase repeat in the chicken genome.
1979,
Pubmed
Hereford,
Isolation of yeast histone genes H2A and H2B.
1979,
Pubmed
Howley,
A rapid method for detecting and mapping homology between heterologous DNAs. Evaluation of polyomavirus genomes.
1979,
Pubmed
Jacob,
Histone-gene reiteration in the genome of mouse.
1976,
Pubmed
Jacob,
Reiteration frequency of the histone genes in the genome of the amphibian, Xenopus laevis.
1976,
Pubmed
,
Xenbase
Jeffreys,
Linkage of adult alpha- and beta-globin genes in X. laevis and gene duplication by tetraploidization.
1980,
Pubmed
,
Xenbase
Kedes,
Reiteration and clustering of DNA sequences complementary to histone messenger RNA.
1971,
Pubmed
Kedes,
Isolation of histone genes from unfractionated sea urchin DNA by subculture cloning in E. coli.
1975,
Pubmed
Kedes,
Histone genes and histone messengers.
1979,
Pubmed
Lifton,
The organization of the histone genes in Drosophila melanogaster: functional and evolutionary implications.
1978,
Pubmed
Moorman,
Histone genes from Xenopus laevis: molecular cloning and initial characterization.
1980,
Pubmed
,
Xenbase
Schaffner,
Genes and spacers of cloned sea urchin histone DNA analyzed by sequencing.
1978,
Pubmed
Southern,
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
1975,
Pubmed
Sures,
The DNA sequence of sea urchin (S. purpuratus) H2A, H2B and H3 histone coding and spacer regions.
1978,
Pubmed
Wahl,
Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate.
1979,
Pubmed
Wienand,
Electrophoretic elution of nucleic acids from gels adapted for subsequent biological tests. Application for analysis of mRNAs from maize endosperm.
1979,
Pubmed
Zernik,
Xenopus laevis histone genes: variant H1 genes are present in different clusters.
1980,
Pubmed
,
Xenbase
