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Nucleotide sequence of the initiation site for ribosomal RNA transcription in Drosophila melanogaster: comparison of genes with and without insertions.
Long EO
,
Rebbert ML
,
Dawid IB
.
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The sequence of 470 nucleotides surrounding the initiation site for rRNA transcription in Drosophila melanogaster has been determined. The precise initiation site was determined first by measuring the DNA fragment protected by the rRNA precursor against digestion by the single-strand specific nuclease S1 and second by direct sequence determination of the first 13 nucleotides of the rRNA precursor. Because greater than 80% od rRNA precursor molecules have been shown previously to bear pppA or ppA 5' termini, we assume that they represent the primary transcription product. Short sequence homologies exist with the initiation regions for rRNA transcription of Xenopus laevis and Saccharomyces cerevisiae. We have determined the nucleotide sequence of the initiation region in four cloned ribosomal genes from D. melanogaster which are not interrupted and in four cloned ribosomal genes in which the 28S rRNA coding region is interrupted by a 5-kilobase type 1 insertion. Three uninterrupted genes and three interrupted genes have identical sequences in the entire analyzed region. The remaining two genes have a single identical base substitution at position -17. We have shown previously that interrupted ribosomal genes in D. melanogaster are not effectively transcribed. Because the nucleotide sequences of the region where transcription initiates are identical in genes with or without insertions, we postulate that the presence of the insertion itself may be responsible for the inactivity of the interrupted genes.
Benton,
Screening lambdagt recombinant clones by hybridization to single plaques in situ.
1977, Pubmed
Benton,
Screening lambdagt recombinant clones by hybridization to single plaques in situ.
1977,
Pubmed
Bogenhagen,
A control region in the center of the 5S RNA gene directs specific initiation of transcription: II. The 3' border of the region.
1980,
Pubmed
,
Xenbase
Corden,
Promoter sequences of eukaryotic protein-coding genes.
1980,
Pubmed
Dawid,
Ribosomal DNA in Drosophila melanogaster. I. Isolation and characterization of cloned fragments.
1978,
Pubmed
Denhardt,
A membrane-filter technique for the detection of complementary DNA.
1966,
Pubmed
Donis-Keller,
Mapping adenines, guanines, and pyrimidines in RNA.
1977,
Pubmed
Glätzer,
Lengths of transcribed rDNA repeating units in spermatocytes of Drosophila hydei: only genes without an intervening sequence are expressed.
1979,
Pubmed
Klemenz,
The 5' terminus of the precursor ribosomal RNA of Saccharomyces cerevisiae.
1980,
Pubmed
Levis,
Processing steps and methylation in the formation of the ribosomal RNA of cultured Drosophila cells.
1978,
Pubmed
Lockard,
Sequence analysis of 5'[32P] labeled mRNA and tRNA using polyacrylamide gel electrophoresis.
1978,
Pubmed
Long,
Repeated genes in eukaryotes.
1980,
Pubmed
,
Xenbase
Long,
Alternative pathways in the processing of ribosomal RNA precursor in Drosophila melanogaster.
1980,
Pubmed
Long,
Restriction analysis of spacers in ribosomal DNA of Drosophila melanogaster.
1979,
Pubmed
Long,
Expression of ribosomal DNA insertions in Drosophila melanogaster.
1979,
Pubmed
Maxam,
Sequencing end-labeled DNA with base-specific chemical cleavages.
1980,
Pubmed
McKnight,
Ultrastructural patterns of RNA synthesis during early embryogenesis of Drosophila melanogaster.
1976,
Pubmed
Pavlakis,
Sequence and secondary structure of Drosophila melanogaster 5.8S and 2S rRNAs and of the processing site between them.
1979,
Pubmed
Perry,
Transcription of mouse kappa chain genes: implications for allelic exclusion.
1980,
Pubmed
Queen,
Computer analysis of nucleic acids and proteins.
1980,
Pubmed
Roiha,
Chracterisation of complete type II insertions in cloned segments of ribosomal DNA from Drosophila melanogaster.
1980,
Pubmed
Sakonju,
A control region in the center of the 5S RNA gene directs specific initiation of transcription: I. The 5' border of the region.
1980,
Pubmed
,
Xenbase
Sanger,
The use of thin acrylamide gels for DNA sequencing.
1978,
Pubmed
Sollner-Webb,
The nucleotide sequence of the initiation and termination sites for ribosomal RNA transcription in X. laevis.
1979,
Pubmed
,
Xenbase
Tomizawa,
Origin of replication of colicin E1 plasmid DNA.
1977,
Pubmed
Valenzuela,
Ribosomal RNA genes of Saccharomyces cerevisiae. II. Physical map and nucleotide sequence of the 5 S ribosomal RNA gene and adjacent intergenic regions.
1977,
Pubmed
Vogt,
Purification and further properties of single-strand-specific nuclease from Aspergillus oryzae.
1973,
Pubmed
Wellauer,
The structural organization of ribosomal DNA in Drosophila melanogaster.
1977,
Pubmed
Wellauer,
X and Y chromosomal ribosomal DNA of Drosophila: comparison of spacers and insertions.
1978,
Pubmed
Wellauer,
Ribosomal DNA in Drosophila melanogaster. II. Heteroduplex mapping of cloned and uncloned rDNA.
1978,
Pubmed
White,
R loop mapping of the 18S and 28S sequences in the long and short repeating units of Drosophila melanogaster rDNA.
1977,
Pubmed
