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Transcription of cloned Xenopus 5S RNA genes by X. laevis RNA polymerase III in reconstituted systems.
Ng SY
,
Parker CS
,
Roeder RG
.
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When incubated with a soluble extract from large oocytes of Xenopus laevis, recombinant DNA plasmids containing either X. laevis oocyte 5S DNA or X. borealis oocyte 5S DNA direct the synthesis of discrete 5S RNAs, which by size and sequence analysis are similar or identical to the corresponding 5S RNAs synthesized in vivo. Synthesis of the 5S RNAs is mediated by a soluble endogenous RNA polymerase III (nucleosidetriphosphate:RNA nucleotidyltransferase, EC 2.7.7.6), which presumably recognizes specific initiation and termination sites in the 5S genes. Optimal conditions for accurate synthesis and the kinetics of the reactions have been determined. A soluble postchromatin supernatant fraction has also been isolated from immature oocytes. Although devoid of a functional endogenous RNA polymerase III, this extract contains a component(s) that effects the accurate transcription of 5S genes (in a plasmid) by a purified RNA polymerase III.
Birkenmeier,
A nuclear extract of Xenopus laevis oocytes that accurately transcribes 5S RNA genes.
1978, Pubmed,
Xenbase
Birkenmeier,
A nuclear extract of Xenopus laevis oocytes that accurately transcribes 5S RNA genes.
1978,
Pubmed
,
Xenbase
Brown,
Cloned single repeating units of 5S DNA direct accurate transcription of 5S RNA when injected into Xenopus oocytes.
1978,
Pubmed
,
Xenbase
Brown,
High-fidelity transcription of 5S DNA injected into Xenopus oocytes.
1977,
Pubmed
,
Xenbase
Brown,
The structure and evolution of ribosomal and 5S DNAs in Xenopus laevis and Xenopus mulleri.
1974,
Pubmed
,
Xenbase
Carroll,
Adjacent repeating units of Xenopus laevis 5S DNA can be heterogeneous in length.
1976,
Pubmed
,
Xenbase
Celma,
Studies of low molecular weight RNA from cells infected with adenovirus 2. II. Heterogeneity at the 5' end of VA-RNA I.
1977,
Pubmed
Denis,
[Biochemical research on oogenesis. V. Comparison between somatic 5 S RNA and oocyte 5 S RNA in Xenopus laevis].
1972,
Pubmed
,
Xenbase
Dumont,
Oogenesis in Xenopus laevis (Daudin). I. Stages of oocyte development in laboratory maintained animals.
1972,
Pubmed
,
Xenbase
Ford,
Sequences of 5S ribosomal RNA from Xenopus mulleri and the evolution of 5S gene-coding sequences.
1976,
Pubmed
,
Xenbase
Harris,
Structural relationships of low molecular weight viral RNAs synthesized by RNA polymerase III in nuclei from adenovirus 2-infected cells.
1978,
Pubmed
Jaehning,
Transcription of specific adenovirus genes in isolated nuclei by exogenous RNA polymerases.
1977,
Pubmed
Korn,
Nucleotide sequence of Xenopus borealis oocyte 5S DNA: comparison of sequences that flank several related eucaryotic genes.
1978,
Pubmed
,
Xenbase
Laskey,
Assembly of SV40 chromatin in a cell-free system from Xenopus eggs.
1977,
Pubmed
,
Xenbase
Miller,
The nucleotide sequence of oocyte 5S DNA in Xenopus laevis. II. The GC-rich region.
1978,
Pubmed
,
Xenbase
Parker,
Faithful gene transcription by eukaryotic RNA polymerases in reconstructed systems.
1978,
Pubmed
Parker,
Selective and accurate transcription of the Xenopus laevis 5S RNA genes in isolated chromatin by purified RNA polymerase III.
1977,
Pubmed
,
Xenbase
Roeder,
Multiple forms of deoxyribonucleic acid-dependent ribonucleic acid polymerase in Xenopus laevis. Levels of activity during oocyte and embryonic development.
1974,
Pubmed
,
Xenbase
Sklar,
Transcription of specific genes in isolated nuclei by exogenous RNA polymerases.
1977,
Pubmed
,
Xenbase
Sklar,
Distinct molecular structures of nuclear class I, II, and III DNA-dependent RNA polymerases.
1975,
Pubmed
,
Xenbase
Woodland,
The synthesis and storage of histones during the oogenesis of Xenopus laevis.
1977,
Pubmed
,
Xenbase
Wu,
Adenovirus DNA-directed transcription of 5.5S RNA in vitro.
1978,
Pubmed
