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A plasmid cloning vehicle allowing regulated expression of eukaryotic DNA in bacteria.
Polisky B
,
Bishop RJ
,
Gelfand DH
.
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We have constructed a plasmid cloning vehicle in which transcription of inserted heterologous DNA fragments can be regulated by a defined bacterial operator and promoter. The lambda plac 5 EcoRIDNA fragment containing the operator, promoter, and beta-galactosidase gene of the lactose operon was linked to the ColE1 derivative plasmid pSF2124, creating a plasmid designated pBGP100, pBGP100 contains one EcoRI site at the lac DNA/pSF2124 DNA junction and another at the lambda DAN/pSF2124 DNA junction. We deleted the latter EcoRI site to generate a plasmid (pBGP120) retaining a single EcoRI site at the lac DNA/nSF2124 DNA junction. To determine whether DNA introduced at the EcoRI site of pBGP120 was expressed under lactose control, we inserted the EcoRI fragment containing 28S ribosomal DNA of Xenopus laevis, creating the hybrid plasmid pBGP123. RNA-DNA hybridization of pulse-labeled RNA from cells containing pBGP123 showed that induction of the lac operon increases the percentage of labeled RNA complementary to Xenopus 28S DNA about 9-fold. This vehicle may be of use for production of eukaryotic gene products in bacteria.
Allet,
Analysis of bacteriophage mu and lambda-mu hybrid DNAs by specific endonucleases.
1975, Pubmed
Allet,
Analysis of bacteriophage mu and lambda-mu hybrid DNAs by specific endonucleases.
1975,
Pubmed
Boyer,
A complementation analysis of the restriction and modification of DNA in Escherichia coli.
1969,
Pubmed
COOK,
Recombination studies of lactose nonfermenting mutants of Escherichia coli K-12.
1962,
Pubmed
Carbon,
Biochemical procedure for production of small deletions in simian virus 40 DNA.
1975,
Pubmed
Chang,
Studies of mouse mitochondrial DNA in Escherichia coli: structure and function of the eucaryotic-procaryotic chimeric plasmids.
1975,
Pubmed
Clewell,
Nature of Col E 1 plasmid replication in Escherichia coli in the presence of the chloramphenicol.
1972,
Pubmed
Cohen,
Construction of biologically functional bacterial plasmids in vitro.
1973,
Pubmed
Hayward,
Anatomy of herpes simplex virus DNA: evidence for four populations of molecules that differ in the relative orientations of their long and short components.
1975,
Pubmed
Helling,
Analysis of endonuclease R-EcoRI fragments of DNA from lambdoid bacteriophages and other viruses by agarose-gel electrophoresis.
1974,
Pubmed
Hershfield,
Plasmid ColEl as a molecular vehicle for cloning and amplification of DNA.
1974,
Pubmed
Kedes,
Isolation of histone genes from unfractionated sea urchin DNA by subculture cloning in E. coli.
1975,
Pubmed
Korn,
Polarity suppressors increase expression of the wild-type tryptophan operon of Escherichia coli.
1976,
Pubmed
Lobban,
Enzymatic end-to end joining of DNA molecules.
1973,
Pubmed
Morrow,
Replication and transcription of eukaryotic DNA in Escherichia coli.
1974,
Pubmed
,
Xenbase
Rabbitts,
Bacterial cloning of plasmids carrying copies of rabbit globin messenger RNA.
1976,
Pubmed
Radding,
Regulation of lambda exonuclease. I. Properties of lambda exonuclease purified from lysogens of lambda T11 and wild type.
1966,
Pubmed
Richardson,
Transcription termination factor rho activity is altered in Escherichia coli with suA gene mutations.
1975,
Pubmed
Rougeon,
Insertion of a rabbit beta-globin gene sequence into an E. coli plasmid.
1975,
Pubmed
Shapiro,
Isolation of pure lac operon DNA.
1969,
Pubmed
So,
The generation of a ColE1-Apr cloning vehicle which allows detection of inserted DNA.
1975,
Pubmed
Struhl,
Functional genetic expression of eukaryotic DNA in Escherichia coli.
1976,
Pubmed
Thomas,
Viable molecular hybrids of bacteriophage lambda and eukaryotic DNA.
1974,
Pubmed
VOGEL,
Acetylornithinase of Escherichia coli: partial purification and some properties.
1956,
Pubmed
Varmus,
Regulation of lac messenger ribonucleic acid synthesis by cyclic adenosine 3',5'-monophosphate and glucose.
1970,
Pubmed
