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The presence of (dA.dT)20-25 tracts in the DNA of primitive eukaryotes.
Mol JN
,
Flavell RA
,
Borst P
.
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Previous work by Jacobson et al. (1) has shown that the number and distribution of (dA.dT)25 tracts in the nuclear DNA of the slime mold Dictyostelium discoideum reflects the number and the distribution of transcriptional units. To investigate whether this is a general phenomenon we compared the nuclear DNAs of other primitive eukaryotes with respect to their content of large (dA.dT)n tracts via the thermal stability of their hybrids with poly(rA). The results of our analysis indicate that all nuclear DNAs tested have (dA.dT)20-25 tracts, but the frequency of such tracts varies from one per 5.4 X 10(6) daltons in Dictyostelium nuclear DNA to one per 2.8 X 10(8) daltons in Crithidia luciliae nuclear DNA. We conclude that the presence of (dA.dT)20-25 tracts is not an obligatory characteristic of the transcriptional unit in primitive eukaryotes. Chromatography of native DNAs on poly(rU) Sephadex columns shows that the large (dA.dT)n tracts occurring in the genomes of both primitive and higher eukaryotes are widely distributed throught these genomes.
Birnboim,
Analysis of long pyrimidine polynucleotides in HeLa cell nuclear DNA: absence of polydeoxythymidylate.
1973, Pubmed
Birnboim,
Analysis of long pyrimidine polynucleotides in HeLa cell nuclear DNA: absence of polydeoxythymidylate.
1973,
Pubmed
Birnboim,
Polypyrimidine segments in Drosophila melanogaster DNA: I. Detection of a cryptic satellite containing polypyrimidine/polypurine DNA.
1975,
Pubmed
Bishop,
Polynucleotide sequences in eukaryotic DNA and RNA that form ribonuclease-resistant complexes with polyuridylic acid.
1974,
Pubmed
Dubroff,
Molecular classes of heterogeneous nuclear RNA in sea urchin embryos.
1975,
Pubmed
Firtel,
A small nuclear precursor of messenger RNA in the cellular slime mold Dictyostelium discoideum.
1973,
Pubmed
Firtel,
Characterization of the genome of the cellular slime mold Dictyostelium discoideum.
1972,
Pubmed
Firtel,
Structural organization and processing of the genetic transcript in the cellular slime mold Dictyostelium discoideum.
1976,
Pubmed
Firtel,
Ribonucleoprotein particles containing heterogeneous nuclear RNA in the cellular slime mold Dictyostelium discoideum.
1975,
Pubmed
Flavell,
DNA-DNA hybridization on nitrocellulose filters. 1. General considerations and non-ideal kinetics.
1974,
Pubmed
Flavell,
The isolation of duplex DNA containing (dA-dT) clusters by affinity chromatography on poly (U) sephadex.
1975,
Pubmed
Fouquet,
Reassociation kinetics of nuclear DNA from Physarum polycephalum.
1974,
Pubmed
Freifelder,
Molecular weights of coliphages and coliphage DNA. IV. Molecular weights of DNA from bacteriophages T4, T5 and T7 and the general problem of determination of M.
1970,
Pubmed
Hollenberg,
Mitochondrial DNA. V. A 25 micron closed circular duplex DNA molecule in wild-type yeast mitochondria. Stucture and genetic complexity.
1970,
Pubmed
Jacobson,
Transcription of polydeoxythymidylate sequences in the genome of the cellular slime mold, Dictyostelium discoideum.
1974,
Pubmed
Kleisen,
Sequence heterogeneity of the mini-circles of kinetoplast DNA of Crithidia luciliae and evidence for the presence of a component more complex than mini-circle DNA in the kinetoplast network.
1975,
Pubmed
Laurent,
Kinetoplast DNA. A unique macromolecular structure of considerable size and mechanical resistance.
1971,
Pubmed
Marshall,
Poly U tracts absent from viral RNA.
1972,
Pubmed
Mol,
The binding of poly (rA) and poly (rU) to denatured DNA. II. Studies with natural DNAs.
1976,
Pubmed
Nakazato,
Localization of the polyadenylate sequences in messenger ribonucleic acid and in the heterogeneous nuclear ribonucleic acid of HeLa cells.
1973,
Pubmed
Pero,
Hybridization of polyuridylic acid to human DNA immobilized onto nitrocellulose filters.
1975,
Pubmed
Sanders,
Nature of the base sequence conserved in the mitochondrial DNA of a low-density petite.
1973,
Pubmed
Sanders,
The organization of genes in yeast mitochondrial DNA II. The physical map of EcoRI and HindII + III fragments.
1975,
Pubmed
Shenkin,
Deoxyadenylate-rich and deoxyguanylate-rich regions in mammalian DNA.
1974,
Pubmed
Steinert,
Specific detection of kinetoplast DNA in cytological preparations of trypanosomes by hybridization with complementary RNA.
1973,
Pubmed
Steinert,
[Autoradiographic study of the effects of ethidium bromide on the synthesis of nucleic acids in Crithidia luciliae].
1969,
Pubmed
Yamamoto,
Rapid bacteriophage sedimentation in the presence of polyethylene glycol and its application to large-scale virus purification.
1970,
Pubmed
