Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
In Vitro
1976 Jun 01;126:418-27. doi: 10.1007/bf02806021.
Show Gene links
Show Anatomy links
Defined nutrient medium for the in vitro maintenance of Xenopus laevis oocytes.
Eppig JJ
,
Dumont JN
.
???displayArticle.abstract???
A procedure is described for the isolation and culture of large numbers of follicle cell-free Xenopus laevis oocytes in all stages of development. The isolation procedure involves the incubation of pieces of ovary in a calcium-free solution OR2 containing 0.2% collagenase. A defined nutrient medium for the maintenace of the oocytes in vitro is presented. It is shown that this medium, referred to as DNOM, can maintain certain morpological and functional characteristics of oocytes for periods up to 3 weeks.
Bramhall,
A simple colorimetric method for determination of protein.
1969, Pubmed
Bramhall,
A simple colorimetric method for determination of protein.
1969,
Pubmed
Colman,
Synthesis of RNA in oocytes of Xenopus laevis during culture in vitro.
1974,
Pubmed
,
Xenbase
Dumont,
Oogenesis in Xenopus laevis (Daudin). I. Stages of oocyte development in laboratory maintained animals.
1972,
Pubmed
,
Xenbase
EAGLE,
Amino acid metabolism in mammalian cell cultures.
1959,
Pubmed
Eppig,
Amino acid pools in developing oocytes of Xenopus laevis.
1972,
Pubmed
,
Xenbase
Eppig,
Comparison of exogenous energy sources for in vitro maintenance of follicle cell-free Xenopus laevis oocytes.
1976,
Pubmed
,
Xenbase
Gurdon,
Changes in somatic cell nuclei inserted into growing and maturing amphibian oocytes.
1968,
Pubmed
,
Xenbase
Hallberg,
Ribosomal protein synthesis in Xenopus laevis oocytes.
1975,
Pubmed
,
Xenbase
Jared,
Protein uptake in vitro by amphibian oocytes.
1969,
Pubmed
,
Xenbase
Mairy,
[Biochemical studies on oogenesis. I. RNA synthesis and accumulation during oogenesis of the South African toad Xenopus laevis].
1971,
Pubmed
,
Xenbase
Mayhew,
Reversibility of ouabain induced inhibition of cell division and cation transport in Ehrlich ascites cells.
1968,
Pubmed
Mott,
An electron-microscope study of the lampbrush chromosomes of the newt Triturus cristatus.
1975,
Pubmed
Roeder,
Multiple forms of deoxyribonucleic acid-dependent ribonucleic acid polymerase in Xenopus laevis. Levels of activity during oocyte and embryonic development.
1974,
Pubmed
,
Xenbase
Wallace,
Protein incorporation by isolated amphibian oocytes. I. Preliminary studies.
1970,
Pubmed
Wallace,
Protein incorporation by isolated amphibian oocytes. 3. Optimum incubation conditions.
1973,
Pubmed
,
Xenbase
Wallace,
The induced synthesis and transport of yolk proteins and their accumulation by the oocyte in Xenopus laevis.
1968,
Pubmed
,
Xenbase
