Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Purification and some characteristics of 5S DNA from Xenopus laevis.
Brown DD
,
Wensink PC
,
Jordan E
.
???displayArticle.abstract???
DNA containing the multiple genes for 5S RNA has been isolated from the genome of Xenopus laevis. Whereas 5S RNA is about 57% G + C, the 5S DNA has a base composition of about 33-35% GC and consists of two alternating regions that differ in base composition by at least 20% GC. A denaturation map of 5S DNA analyzed by electron microscopy demonstrates that the repeating pattern is regular and each repeating unit has a mass of about 500,000 daltons. If one gene for 5S RNA (84,000 daltons native) were present in each repeat, it should comprise about 16.8% of 5S DNA. This arrangement is confirmed, since 6.8% of pure 5S DNA (13.6% of its base pairs) hybridized with 5S RNA. The remaining 83% of each repeating unit is considered to be "spacer" DNA. The 5S RNA hybridizes with about 0.05% of the bulk DNA of X. laevis, so that 5S DNA comprises about 0.7% of the total nuclear DNA. This is equivalent to about 24,000 repeating units for each haploid complement of DNA. These repeats are highly clustered; as many as 86 have been visualized along a single DNA molecule.
Aloni,
Studies of fractionated HeLa cell metaphase chromosomes. II. chromosomal distribution of sites for transfer RNA and 5 s RNA.
1971, Pubmed
Aloni,
Studies of fractionated HeLa cell metaphase chromosomes. II. chromosomal distribution of sites for transfer RNA and 5 s RNA.
1971,
Pubmed
Birnstiel,
Localization of the ribosomal DNA complements in the nucleolar organizer region of Xenopus laevis.
1966,
Pubmed
,
Xenbase
Brown,
Synthesis and accumulation of low molecular weight RNA during embryogenesis of Xenopus laevis.
1966,
Pubmed
,
Xenbase
Brown,
Gene linkage by RNA-DNA hybridization. I. Unique DNA sequences homologous to 4 s RNA, 5 s RNA and ribosomal RNA.
1968,
Pubmed
,
Xenbase
Corneo,
Isolation and characterization of mouse and guinea pig satellite deoxyribonucleic acids.
1968,
Pubmed
Corneo,
Different satellite deoxyribonucleic acids of guinea pig and ox.
1970,
Pubmed
Corneo,
Repeated sequences in human DNA.
1970,
Pubmed
Dawid,
Composition and structure of chromosomal and amplified ribosomal DNA's of Xenopus laevis.
1970,
Pubmed
,
Xenbase
Freifelder,
Molecular weights of coliphages and coliphage DNA. IV. Molecular weights of DNA from bacteriophages T4, T5 and T7 and the general problem of determination of M.
1970,
Pubmed
Hatlen,
Oligonucleotide pattern after pancreatic ribonuclease digestion and the 3' and 5' termini of 5S ribonucleic acid from HeLa cells.
1969,
Pubmed
Kersten,
Physicochemical properties of complexes between deoxyribonucleic acid and antibiotics which affect ribonucleic acid synthesis (actinomycin, daunomycin, cinerubin, nogalamycin, chormomycin, mithramycin, and olivomycin).
1966,
Pubmed
Kirk,
Effect of methylation of cytosine residues on the buoyant density of DNA in caesium chloride solution.
1967,
Pubmed
Mairy,
[Biochemical studies on oogenesis. I. RNA synthesis and accumulation during oogenesis of the South African toad Xenopus laevis].
1971,
Pubmed
,
Xenbase
McConaughy,
Nucleic acid reassociation in formamide.
1969,
Pubmed
Perry,
Persistent synthesis of 5S RNA when production of 28S and 18S ribosomal RNA is inhibited by low doses of actinomycin D.
1968,
Pubmed
SCHILDKRAUT,
Determination of the base composition of deoxyribonucleic acid from its buoyant density in CsCl.
1962,
Pubmed
Smith,
The introduction of radioisotopes into RNA by methylation in vitro.
1967,
Pubmed
Wensink,
Denaturation map of the ribosomal DNA of Xenopus laevis.
1971,
Pubmed
,
Xenbase
Wimber,
Localization of 5S RNA genes on Drosophila chromosomes by RNA-DNA hybridization.
1970,
Pubmed
