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Putrescine and spermidine biosynthesis in the development of normal and anucleolate mutants of Xenopus laevis.
Russell DH
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Ornithine decarboxylase (EC 4.1.1.17), the enzyme that catalyzes the synthesis of putrescine from ornithine, increases dramatically in developing Xenopus embryos. Between the 2-cell stage and early blastula stage, activity increases 10-fold, and in swimming tadpoles, the enzyme activity is 100-fold higher than that present in either unfertilized eggs or 2-cell embryos. S-adenosyl-L-methionine decarboxylase, an enzyme that catalyzes spermidine synthesis from putrescine and S-adenosyl-L-methionine, increases 40-fold in activity during the development of Xenopus, but does not increase in activity prior to gastrulation. Concomitant with these enzyme changes, putrescine and spermidine concentrations are elevated during the development of Xenopus embryos. Maximal accumulations are present in the swimming tadpole and correspond to maximal enzyme activities. Anucleolate-mutant embryos of Xenopus, which do not synthesize new ribosomes, have no detectable S-adenosyl-L-methionine decarboxylase activity and do not accumulate spermidine after gastrulation. Ornithine decarboxylase activity is depressed in these mutants and putrescine accumulation is decreased also. The activity of some dehydrogenases that increase in Xenopus embryos after gastrulation show normal increases in the anucleolate mutants. Thus, the synthesis of putrescine and spermidine in embryos correlates with the onset of ribosomal-RNA synthesis and the formation of a viable nucleolus in the embryonic cell.
Abraham,
Studies on DNA-dependent RNA polymerase from Escherichia coli. 1. The mechanism of polyamine induced stimulation of enzyme activity.
1968, Pubmed
Abraham,
Studies on DNA-dependent RNA polymerase from Escherichia coli. 1. The mechanism of polyamine induced stimulation of enzyme activity.
1968,
Pubmed
BROWN,
ABSENCE OF RIBOSOMAL RNA SYNTHESIS IN THE ANUCLEOLATE MUTANT OF XENOPUS LAEVIS.
1964,
Pubmed
,
Xenbase
Caldarera,
Polyamines and nucleic acids during development of the chick embryo.
1965,
Pubmed
Caldarera,
Spermine and spermidine of the prostate gland of orchiectomized rats and their effect on RNA polymerase activity.
1968,
Pubmed
Dykstra,
Spermidine in Regenerating Liver: Relation to Rapid Synthesis of Ribonucleic Acid.
1965,
Pubmed
ELSDALE,
A mutation that reduces nucleolar number in Xenopus laevis.
1958,
Pubmed
,
Xenbase
HEILMANN,
[Amino acid determination on paper chromatograms].
1957,
Pubmed
JAENNE,
SPERMIDINE AND SPERMINE IN RAT TISSUES AT DIFFERENT AGES.
1964,
Pubmed
Jänne,
Studies on the biosynthetic pathway of polyamines in rat liver.
1967,
Pubmed
Jänne,
Stimulation of spermidine synthesis in the regenerating rat liver: relation to increased ornithine decarboxylase activity.
1968,
Pubmed
Jänne,
Mammalian ornithine decarboxylase: activation and alteration of physical behaviour by thiol compounds.
1970,
Pubmed
Kostyo,
Changes in polyamine content of rat liver following hypophysectomy and treatment with growth hormone.
1966,
Pubmed
LOWRY,
Protein measurement with the Folin phenol reagent.
1951,
Pubmed
Lievens,
RNA polymerase activity during development of normal and hybrid amphibian embryos.
1970,
Pubmed
Pegg,
On the role of S-adenosyl-L-methionine in the biosynthesis of spermidine by rat prostate.
1969,
Pubmed
Raina,
Stimulation of polyamine synthesis in relation to nucleic acids in regenerating rat liver.
1966,
Pubmed
Raina,
Association of polyamines and RNA in isolated subcellular particles from rat liver.
1967,
Pubmed
Russell,
Amine synthesis in regenerating rat liver: extremely rapid turnover of ornithine decarboxylase.
1969,
Pubmed
Russell,
The dynamics of synthesis and degradation of polyamines in normal and regenerating rat liver and brain.
1970,
Pubmed
Russell,
Amine synthesis in regenerating rat liver: effect of hypophysectomy and growth hormone on ornithine decarboxylase.
1969,
Pubmed
Russell,
Amine synthesis in rapidly growing tissues: ornithine decarboxylase activity in regenerating rat liver, chick embryo, and various tumors.
1968,
Pubmed
Russell,
Growth hormone induction of ornithine decarboxylase in rat liver.
1970,
Pubmed
Russell,
Presence and biosynthesis of putrescine and polyamines in amphibians.
1969,
Pubmed
TABOR,
SPERMIDINE, SPERMINE, AND RELATED AMINES.
1964,
Pubmed
Wallace,
Ribosomal cistrons and the nucleolar organizer.
1966,
Pubmed