Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Myosin heavy chain isoform composition and stretch activation kinetics in single fibres of Xenopus laevis iliofibularismuscle.
Andruchova O
,
Stephenson GM
,
Andruchov O
,
Stephenson DG
,
Galler S
.
???displayArticle.abstract???
Skeletal muscle is composed of specialized fibre types that enable it to fulfil complex and variable functional needs. Muscle fibres of Xenopus laevis, a frog formerly classified as a toad, were the first to be typed based on a combination of physiological, morphological, histochemical and biochemical characteristics. Currently the most widely accepted criterion for muscle fibre typing is the myosin heavy chain (MHC) isoform composition because it is assumed that variations of this protein are the most important contributors to functional diversity. Yet this criterion has not been used for classification of Xenopus fibres due to the lack of an effective protocol for MHC isoform analysis. In the present study we aimed to resolve and visualize electrophoretically the MHC isoforms expressed in the iliofibularis muscle of Xenopus laevis, to define their functional identity and to classify the fibres based on their MHC isoform composition. Using a SDS-PAGE protocol that proved successful with mammalian muscle MHC isoforms, we were able to detect five MHC isoforms in Xenopus iliofibularis muscle. The kinetics of stretch-induced force transients (stretch activation) produced by a fibre was strongly correlated with its MHC isoform content indicating that the five MHC isoforms confer different kinetics characteristics. Hybrid fibre types containing two MHC isoforms exhibited stretch activation kinetics parameters that were intermediate between those of the corresponding pure fibre types. These results clearly show that the MHC isoforms expressed in Xenopus muscle are functionally different thereby validating the idea that MHC isoform composition is the most reliable criterion for vertebrate skeletal muscle fibre type classification. Thus, our results lay the foundation for the unequivocal classification of the muscle fibres in the Xenopus iliofibularis muscle and for gaining further insights into skeletal muscle fibre diversity.
Andruchov,
Kinetic properties of myosin heavy chain isoforms in mouse skeletal muscle: comparison with rat, rabbit, and human and correlation with amino acid sequence.
2004, Pubmed
Andruchov,
Kinetic properties of myosin heavy chain isoforms in mouse skeletal muscle: comparison with rat, rabbit, and human and correlation with amino acid sequence.
2004,
Pubmed
Andruchov,
Dependence of cross-bridge kinetics on myosin light chain isoforms in rabbit and rat skeletal muscle fibres.
2006,
Pubmed
Andruchov,
Functional properties of skinned rabbit skeletal and cardiac muscle preparations containing alpha-cardiac myosin heavy chain.
2004,
Pubmed
Bottinelli,
Functional heterogeneity of mammalian single muscle fibres: do myosin isoforms tell the whole story?
2001,
Pubmed
Ford,
Tension responses to sudden length change in stimulated frog muscle fibres near slack length.
1977,
Pubmed
Galler,
Ca2+_, Sr2+_force relationships and kinetic properties of fast-twitch rat leg muscle fibre subtypes.
1999,
Pubmed
Galler,
Stretch activation of skeletal muscle fibre types.
1994,
Pubmed
Galler,
Stretch activation, unloaded shortening velocity, and myosin heavy chain isoforms of rat skeletal muscle fibres.
1994,
Pubmed
Galler,
Unloaded shortening of skinned mammalian skeletal muscle fibres: effects of the experimental approach and passive force.
1994,
Pubmed
Galler,
Force responses following stepwise length changes of rat skeletal muscle fibre types.
1996,
Pubmed
Galler,
Stretch activation and myosin heavy chain isoforms of rat, rabbit and human skeletal muscle fibres.
1997,
Pubmed
Galler,
Elementary steps of the cross-bridge cycle in fast-twitch fiber types from rabbit skeletal muscles.
2005,
Pubmed
Gordon,
The variation in isometric tension with sarcomere length in vertebrate muscle fibres.
1966,
Pubmed
Heinl,
Tension responses to quick length changes of glycerinated skeletal muscle fibres from the frog and tortoise.
1974,
Pubmed
Hilber,
Kinetic properties of myosin heavy chain isoforms in single fibers from human skeletal muscle.
1999,
Pubmed
Hilber,
Improvement of the measurements on skinned muscle fibres by fixation of the fibre ends with glutaraldehyde.
1998,
Pubmed
Horiuti,
Some properties of the contractile system and sarcoplasmic reticulum of skinned slow fibres from Xenopus muscle.
1986,
Pubmed
,
Xenbase
Huxley,
Mechanics and models of the myosin motor.
2000,
Pubmed
Huxley,
Proposed mechanism of force generation in striated muscle.
1971,
Pubmed
Kawai,
Sinusoidal analysis: a high resolution method for correlating biochemical reactions with physiological processes in activated skeletal muscles of rabbit, frog and crayfish.
1980,
Pubmed
Kawai,
Cross-bridge scheme and force per cross-bridge state in skinned rabbit psoas muscle fibers.
1993,
Pubmed
Lutz,
Identification of myosin light chains in Rana pipiens skeletal muscle and their expression patterns along single fibres.
2001,
Pubmed
Lutz,
Four novel myosin heavy chain transcripts define a molecular basis for muscle fibre types in Rana pipiens.
1998,
Pubmed
Lutz,
Quantitative analysis of muscle fibre type and myosin heavy chain distribution in the frog hindlimb: implications for locomotory design.
1998,
Pubmed
Lännergren,
The force-velocity relation of isolated twitch and slow muscle fibres of Xenopus laevis.
1978,
Pubmed
,
Xenbase
Lännergren,
An intermediate type of muscle fibre in Xenopus laevis.
1979,
Pubmed
,
Xenbase
Lännergren,
Myosin isoenzymes in single muscle fibres of Xenopus laevis: analysis of five different functional types.
1984,
Pubmed
,
Xenbase
Lännergren,
Contractile properties and myosin isoenzymes of various kinds of Xenopus twitch muscle fibres.
1987,
Pubmed
,
Xenbase
Nasledov,
[Study of the contractile mechanism of frog tonic muscle fibers].
1971,
Pubmed
Nguyen,
An electrophoretic study of myosin heavy chain expression in skeletal muscles of the toad Bufo marinus.
1999,
Pubmed
Nguyen,
Myosin heavy chain isoform expression and Ca2 +-stimulated ATPase activity in single fibres of toad rectus abdominis muscle.
2002,
Pubmed
O'Connell,
Electrophoretic and functional identification of two troponin C isoforms in toad skeletal muscle fibers.
2006,
Pubmed
Pette,
Cellular and molecular diversities of mammalian skeletal muscle fibers.
1990,
Pubmed
Schiaffino,
Molecular diversity of myofibrillar proteins: gene regulation and functional significance.
1996,
Pubmed
Smith,
Types of motor units in the skeletal muscle of Xenopus laevis.
1968,
Pubmed
,
Xenbase
Smith,
Varieties of fast and slow extrafusal muscle fibres in amphibian hind limb muscles.
1973,
Pubmed
,
Xenbase
Spurway,
Quantitative analysis of histochemical and immunohistochemical reactions in skeletal muscle fibres of Rana and Xenopus.
1989,
Pubmed
,
Xenbase
Stephenson,
Hybrid skeletal muscle fibres: a rare or common phenomenon?
2001,
Pubmed
Stephenson,
Calcium-activated force responses in fast- and slow-twitch skinned muscle fibres of the rat at different temperatures.
1981,
Pubmed
Stienen,
ATP utilization for calcium uptake and force production in skinned muscle fibres of Xenopus laevis.
1995,
Pubmed
,
Xenbase
van der Laarse,
Calcium-stimulated myofibrillar ATPase activity correlates with shortening velocity of muscle fibres in Xenopus laevis.
1986,
Pubmed
,
Xenbase
