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Dissection of the unusual structural and functional properties of the variant H2A.Bbd nucleosome.
Doyen CM
,
Montel F
,
Gautier T
,
Menoni H
,
Claudet C
,
Delacour-Larose M
,
Angelov D
,
Hamiche A
,
Bednar J
,
Faivre-Moskalenko C
,
Bouvet P
,
Dimitrov S
.
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The histone variant H2A.Bbd appeared to be associated with active chromatin, but how it functions is unknown. We have dissected the properties of nucleosome containing H2A.Bbd. Atomic force microscopy (AFM) and electron cryo-microscopy (cryo-EM) showed that the H2A.Bbd histone octamer organizes only approximately 130 bp of DNA, suggesting that 10 bp of each end of nucleosomal DNA are released from the octamer. In agreement with this, the entry/exit angle of the nucleosomal DNA ends formed an angle close to 180 degrees and the physico-chemical analysis pointed to a lower stability of the variant particle. Reconstitution of nucleosomes with swapped-tail mutants demonstrated that the N-terminus of H2A.Bbd has no impact on the nucleosome properties. AFM, cryo-EM and chromatin remodeling experiments showed that the overall structure and stability of the particle, but not its property to interfere with the SWI/SNF induced remodeling, were determined to a considerable extent by the H2A.Bbd docking domain. These data show that the whole H2A.Bbd histone fold domain is responsible for the unusual properties of the H2A.Bbd nucleosome.
Abbott,
Characterization of the stability and folding of H2A.Z chromatin particles: implications for transcriptional activation.
2001, Pubmed
Abbott,
Characterization of the stability and folding of H2A.Z chromatin particles: implications for transcriptional activation.
2001,
Pubmed
Ahmad,
Epigenetic consequences of nucleosome dynamics.
2002,
Pubmed
Angelov,
The histone variant macroH2A interferes with transcription factor binding and SWI/SNF nucleosome remodeling.
2003,
Pubmed
Angelov,
Nucleolin is a histone chaperone with FACT-like activity and assists remodeling of nucleosomes.
2006,
Pubmed
,
Xenbase
Angelov,
SWI/SNF remodeling and p300-dependent transcription of histone variant H2ABbd nucleosomal arrays.
2004,
Pubmed
,
Xenbase
Ausió,
The many tales of a tail: carboxyl-terminal tail heterogeneity specializes histone H2A variants for defined chromatin function.
2002,
Pubmed
Bao,
Nucleosomes containing the histone variant H2A.Bbd organize only 118 base pairs of DNA.
2004,
Pubmed
Bassing,
Histone H2AX: a dosage-dependent suppressor of oncogenic translocations and tumors.
2003,
Pubmed
Beato,
Transcription factor access to chromatin.
1997,
Pubmed
Becker,
Nucleosome sliding: facts and fiction.
2002,
Pubmed
Bednar,
Cryoelectron microscopic analysis of nucleosomes and chromatin.
1999,
Pubmed
Bednar,
Chromatin conformation and salt-induced compaction: three-dimensional structural information from cryoelectron microscopy.
1995,
Pubmed
Celeste,
H2AX haploinsufficiency modifies genomic stability and tumor susceptibility.
2003,
Pubmed
Chadwick,
A novel chromatin protein, distantly related to histone H2A, is largely excluded from the inactive X chromosome.
2001,
Pubmed
Chadwick,
Histone variant macroH2A contains two distinct macrochromatin domains capable of directing macroH2A to the inactive X chromosome.
2001,
Pubmed
Claudet,
Histone octamer instability under single molecule experiment conditions.
2005,
Pubmed
Costanzi,
Histone macroH2A1 is concentrated in the inactive X chromosome of female mammals.
1998,
Pubmed
Costanzi,
MACROH2A2, a new member of the MARCOH2A core histone family.
2001,
Pubmed
Côté,
Stimulation of GAL4 derivative binding to nucleosomal DNA by the yeast SWI/SNF complex.
1994,
Pubmed
Dhillon,
A histone variant, Htz1p, and a Sir1p-like protein, Esc2p, mediate silencing at HMR.
2000,
Pubmed
Doyen,
Mechanism of polymerase II transcription repression by the histone variant macroH2A.
2006,
Pubmed
,
Xenbase
Faast,
Histone variant H2A.Z is required for early mammalian development.
2001,
Pubmed
Gautier,
Histone variant H2ABbd confers lower stability to the nucleosome.
2004,
Pubmed
Henikoff,
Assembly of variant histones into chromatin.
2005,
Pubmed
Henikoff,
Histone variants, nucleosome assembly and epigenetic inheritance.
2004,
Pubmed
Kamakaka,
Histone variants: deviants?
2005,
Pubmed
Karras,
The macro domain is an ADP-ribose binding module.
2005,
Pubmed
Kulić,
DNA spools under tension.
2004,
Pubmed
Kustatscher,
Splicing regulates NAD metabolite binding to histone macroH2A.
2005,
Pubmed
Luger,
Expression and purification of recombinant histones and nucleosome reconstitution.
1999,
Pubmed
Luger,
Crystal structure of the nucleosome core particle at 2.8 A resolution.
1997,
Pubmed
Lyubchenko,
Atomic force microscopy of DNA and bacteriophage in air, water and propanol: the role of adhesion forces.
1993,
Pubmed
Mermoud,
Histone macroH2A1.2 relocates to the inactive X chromosome after initiation and propagation of X-inactivation.
1999,
Pubmed
Mutskov,
Persistent interactions of core histone tails with nucleosomal DNA following acetylation and transcription factor binding.
1998,
Pubmed
Neely,
Activation domain-mediated targeting of the SWI/SNF complex to promoters stimulates transcription from nucleosome arrays.
1999,
Pubmed
Pehrson,
MacroH2A, a core histone containing a large nonhistone region.
1992,
Pubmed
Perche,
Higher concentrations of histone macroH2A in the Barr body are correlated with higher nucleosome density.
2000,
Pubmed
Rangasamy,
RNA interference demonstrates a novel role for H2A.Z in chromosome segregation.
2004,
Pubmed
Redon,
Histone H2A variants H2AX and H2AZ.
2002,
Pubmed
Rogakou,
DNA double-stranded breaks induce histone H2AX phosphorylation on serine 139.
1998,
Pubmed
Santisteban,
Histone H2A.Z regulats transcription and is partially redundant with nucleosome remodeling complexes.
2000,
Pubmed
Sarma,
Histone variants meet their match.
2005,
Pubmed
Strahl,
The language of covalent histone modifications.
2000,
Pubmed
Suto,
Crystal structure of a nucleosome core particle containing the variant histone H2A.Z.
2000,
Pubmed
,
Xenbase
Van Holde,
DNA-histone interactions in nucleosomes.
1980,
Pubmed
