Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Proc Natl Acad Sci U S A
2006 Nov 21;10347:17801-6. doi: 10.1073/pnas.0608484103.
Show Gene links
Show Anatomy links
ELYS is a dual nucleoporin/kinetochore protein required for nuclear pore assembly and proper cell division.
Rasala BA
,
Orjalo AV
,
Shen Z
,
Briggs S
,
Forbes DJ
.
???displayArticle.abstract???
Nuclear pores span the nuclear envelope and act as gated aqueous channels to regulate the transport of macromolecules between the nucleus and cytoplasm, from individual proteins and RNAs to entire viral genomes. By far the largest subunit of the nuclear pore is the Nup107-160 complex, which consists of nine proteins and is critical for nuclear pore assembly. At mitosis, the Nup107-160 complex localizes to kinetochores, suggesting that it may also function in chromosome segregation. To investigate the dual roles of the Nup107-160 complex at the pore and during mitosis, we set out to identify binding partners by immunoprecipitation from both interphase and mitotic Xenopus egg extracts and mass spectrometry. ELYS, a putative transcription factor, was discovered to copurify with the Nup107-160 complex in Xenopus interphase extracts, Xenopus mitotic extracts, and human cell extracts. Indeed, a large fraction of ELYS localizes to the nuclear pore complexes of HeLa cells. Importantly, depletion of ELYS by RNAi leads to severe disruption of nuclear pores in the nuclear envelope, whereas lamin, Ran, and tubulin staining appear normal. At mitosis, ELYS targets to kinetochores, and RNAi depletion from HeLa cells leads to an increase in cytokinesis defects. Thus, we have identified an unexpected member of the nuclear pore and kinetochore that functions in both pore assembly at the nucleus and faithful cell division.
Belgareh,
An evolutionarily conserved NPC subcomplex, which redistributes in part to kinetochores in mammalian cells.
2001, Pubmed
Belgareh,
An evolutionarily conserved NPC subcomplex, which redistributes in part to kinetochores in mammalian cells.
2001,
Pubmed
Bickford,
A structural view of the COPII vesicle coat.
2004,
Pubmed
Blower,
A Rae1-containing ribonucleoprotein complex is required for mitotic spindle assembly.
2005,
Pubmed
,
Xenbase
Bodoor,
Function and assembly of nuclear pore complex proteins.
1999,
Pubmed
Bodoor,
Sequential recruitment of NPC proteins to the nuclear periphery at the end of mitosis.
1999,
Pubmed
Boehmer,
Depletion of a single nucleoporin, Nup107, prevents the assembly of a subset of nucleoporins into the nuclear pore complex.
2003,
Pubmed
Casolari,
Genome-wide localization of the nuclear transport machinery couples transcriptional status and nuclear organization.
2004,
Pubmed
Cronshaw,
Proteomic analysis of the mammalian nuclear pore complex.
2002,
Pubmed
Dilworth,
The mobile nucleoporin Nup2p and chromatin-bound Prp20p function in endogenous NPC-mediated transcriptional control.
2005,
Pubmed
Fernandez,
MEL-28 is downstream of the Ran cycle and is required for nuclear-envelope function and chromatin maintenance.
2006,
Pubmed
Fontoura,
A conserved biogenesis pathway for nucleoporins: proteolytic processing of a 186-kilodalton precursor generates Nup98 and the novel nucleoporin, Nup96.
1999,
Pubmed
Galy,
Nuclear pore complexes in the organization of silent telomeric chromatin.
2000,
Pubmed
Galy,
MEL-28, a novel nuclear-envelope and kinetochore protein essential for zygotic nuclear-envelope assembly in C. elegans.
2006,
Pubmed
Griffis,
Nup98 is a mobile nucleoporin with transcription-dependent dynamics.
2002,
Pubmed
,
Xenbase
Guan,
Nup50, a nucleoplasmically oriented nucleoporin with a role in nuclear protein export.
2000,
Pubmed
Gunsalus,
Predictive models of molecular machines involved in Caenorhabditis elegans early embryogenesis.
2005,
Pubmed
Harel,
Removal of a single pore subcomplex results in vertebrate nuclei devoid of nuclear pores.
2003,
Pubmed
,
Xenbase
Harel,
Importin beta negatively regulates nuclear membrane fusion and nuclear pore complex assembly.
2003,
Pubmed
,
Xenbase
Hope,
Structural and functional characterization of the short acidic transcriptional activation region of yeast GCN4 protein.
1988,
Pubmed
Howman,
Early disruption of centromeric chromatin organization in centromere protein A (Cenpa) null mice.
2000,
Pubmed
Joseph,
SUMO-1 targets RanGAP1 to kinetochores and mitotic spindles.
2002,
Pubmed
Joseph,
The RanGAP1-RanBP2 complex is essential for microtubule-kinetochore interactions in vivo.
2004,
Pubmed
Kimura,
Identification of a novel transcription factor, ELYS, expressed predominantly in mouse foetal haematopoietic tissues.
2002,
Pubmed
Krull,
Nucleoporins as components of the nuclear pore complex core structure and Tpr as the architectural element of the nuclear basket.
2004,
Pubmed
Loïodice,
The entire Nup107-160 complex, including three new members, is targeted as one entity to kinetochores in mitosis.
2004,
Pubmed
Meier,
Nuclear pore complex assembly studied with a biochemical assay for annulate lamellae formation.
1995,
Pubmed
,
Xenbase
Miller,
Identification of a new vertebrate nucleoporin, Nup188, with the use of a novel organelle trap assay.
2000,
Pubmed
,
Xenbase
Moore,
Npap60: a new player in nuclear protein import.
2003,
Pubmed
Okita,
Targeted disruption of the mouse ELYS gene results in embryonic death at peri-implantation development.
2004,
Pubmed
Orjalo,
The Nup107-160 nucleoporin complex is required for correct bipolar spindle assembly.
2006,
Pubmed
,
Xenbase
Reeves,
Molecular biology of HMGA proteins: hubs of nuclear function.
2001,
Pubmed
Ryan,
The nuclear pore complex: a protein machine bridging the nucleus and cytoplasm.
2000,
Pubmed
Salina,
Nup358 integrates nuclear envelope breakdown with kinetochore assembly.
2003,
Pubmed
Shah,
Major binding sites for the nuclear import receptor are the internal nucleoporin Nup153 and the adjacent nuclear filament protein Tpr.
1998,
Pubmed
,
Xenbase
Siniossoglou,
A novel complex of nucleoporins, which includes Sec13p and a Sec13p homolog, is essential for normal nuclear pores.
1996,
Pubmed
Suntharalingam,
Peering through the pore: nuclear pore complex structure, assembly, and function.
2003,
Pubmed
Sönnichsen,
Full-genome RNAi profiling of early embryogenesis in Caenorhabditis elegans.
2005,
Pubmed
Vasu,
Novel vertebrate nucleoporins Nup133 and Nup160 play a role in mRNA export.
2001,
Pubmed
,
Xenbase
Walther,
The conserved Nup107-160 complex is critical for nuclear pore complex assembly.
2003,
Pubmed
,
Xenbase
van Deursen,
G2 arrest and impaired nucleocytoplasmic transport in mouse embryos lacking the proto-oncogene CAN/Nup214.
1996,
Pubmed
