Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
Development. December 1, 2006; 133 (24): 4827-38.

FoxD3 regulation of Nodal in the Spemann organizer is essential for Xenopus dorsal mesoderm development.

Steiner AB , Engleka MJ , Lu Q , Piwarzyk EC , Yaklichkin S , Lefebvre JL , Walters JW , Pineda-Salgado L , Labosky PA , Kessler DS .

Induction and patterning of the mesodermal germ layer is a key early step of vertebrate embryogenesis. We report that FoxD3 function in the Xenopus gastrula is essential for dorsal mesodermal development and for Nodal expression in the Spemann organizer. In embryos and explants, FoxD3 induced mesodermal genes, convergent extension movements and differentiation of axial tissues. Engrailed-FoxD3, but not VP16-FoxD3, was identical to native FoxD3 in mesoderm-inducing activity, indicating that FoxD3 functions as a transcriptional repressor to induce mesoderm. Antagonism of FoxD3 with VP16-FoxD3 or morpholino-knockdown of FoxD3 protein resulted in a complete block to axis formation, a loss of mesodermal gene expression, and an absence of axial mesoderm, indicating that transcriptional repression by FoxD3 is required for mesodermal development. FoxD3 induced mesoderm in a non-cell-autonomous manner, indicating a role for secreted inducing factors in the response to FoxD3. Consistent with this mechanism, FoxD3 was necessary and sufficient for the expression of multiple Nodal-related genes, and inhibitors of Nodal signaling blocked mesoderm induction by FoxD3. Therefore, FoxD3 is required for Nodal expression in the Spemann organizer and this function is essential for dorsal mesoderm formation.

PubMed ID: 17092955
PMC ID: PMC1676154
Article link: Development.
Grant support: GM64768 NIGMS NIH HHS , HD36720 NICHD NIH HHS , R01 GM064768-01 NIGMS NIH HHS , R01 GM064768-02 NIGMS NIH HHS , R01 GM064768-03 NIGMS NIH HHS , R01 GM064768-04 NIGMS NIH HHS , R01 GM064768 NIGMS NIH HHS , R01 HD036720 NICHD NIH HHS

Genes referenced: act3 actl6a cer1 chrd.1 dlx3 foxd3 foxh1 gdf3 gsc mapk1 mixer myod1 ncam1 nodal nodal1 nodal2 smad2 tbxt vegt wnt8a zic1

Morpholinos referenced: foxd3 MO2

External Resources:
Article Images: [+] show captions

Agius, 2000, Pubmed, Xenbase[+]

Xenbase: The Xenopus laevis and X. tropicalis resource.
Version: 4.9.0
Major funding for Xenbase is provided by the National Institute of Child Health and Human Development, grant P41 HD064556