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Proc Natl Acad Sci U S A
2006 Aug 15;10333:12365-70. doi: 10.1073/pnas.0602426103.
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The structure of the endoribonuclease XendoU: From small nucleolar RNA processing to severe acute respiratory syndrome coronavirus replication.
Renzi F
,
Caffarelli E
,
Laneve P
,
Bozzoni I
,
Brunori M
,
Vallone B
.
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Small nucleolar RNAs (snoRNAs) play a key role in eukaryotic ribosome biogenesis. In most cases, snoRNAs are encoded in introns and are released through the splicing reaction. Some snoRNAs are, instead, produced by an alternative pathway consisting of endonucleolytic processing of pre-mRNA. XendoU, the endoribonuclease responsible for this activity, is a U-specific, metal-dependent enzyme that releases products with 2'-3' cyclic phosphate termini. XendoU is broadly conserved among eukaryotes, and it is a genetic marker of nidoviruses, including the severe acute respiratory syndrome coronavirus, where it is essential for replication and transcription. We have determined by crystallography the structure of XendoU that, by refined search methodologies, appears to display a unique fold. Based on sequence conservation, mutagenesis, and docking simulations, we have identified the active site. The conserved structural determinants of this site may provide a framework for attempting to design antiviral drugs to interfere with the infectious nidovirus life cycle.
,
The CCP4 suite: programs for protein crystallography.
2004, Pubmed
,
The CCP4 suite: programs for protein crystallography.
2004,
Pubmed
Brünger,
Crystallography & NMR system: A new software suite for macromolecular structure determination.
1998,
Pubmed
Caffarelli,
Processing of the intron-encoded U16 and U18 snoRNAs: the conserved C and D boxes control both the processing reaction and the stability of the mature snoRNA.
1996,
Pubmed
,
Xenbase
Caffarelli,
In vitro study of processing of the intron-encoded U16 small nucleolar RNA in Xenopus laevis.
1994,
Pubmed
,
Xenbase
Emsley,
Coot: model-building tools for molecular graphics.
2004,
Pubmed
Filipowicz,
Biogenesis of small nucleolar ribonucleoproteins.
2002,
Pubmed
Gioia,
Functional characterization of XendoU, the endoribonuclease involved in small nucleolar RNA biosynthesis.
2005,
Pubmed
,
Xenbase
Giorgi,
Release of U18 snoRNA from its host intron requires interaction of Nop1p with the Rnt1p endonuclease.
2001,
Pubmed
Grundmann,
Cloning and expression of a cDNA encoding human placental protein 11, a putative serine protease with diagnostic significance as a tumor marker.
1990,
Pubmed
Holm,
Dali: a network tool for protein structure comparison.
1995,
Pubmed
Ivanov,
Major genetic marker of nidoviruses encodes a replicative endoribonuclease.
2004,
Pubmed
Kurz,
The affinity of magnesium binding sites in the Bacillus subtilis RNase P x pre-tRNA complex is enhanced by the protein subunit.
2002,
Pubmed
Laneve,
Purification, cloning, and characterization of XendoU, a novel endoribonuclease involved in processing of intron-encoded small nucleolar RNAs in Xenopus laevis.
2003,
Pubmed
,
Xenbase
Loria,
Recognition of the T stem-loop of a pre-tRNA substrate by the ribozyme from Bacillus subtilis ribonuclease P.
1997,
Pubmed
McGuffin,
The PSIPRED protein structure prediction server.
2000,
Pubmed
Murshudov,
Refinement of macromolecular structures by the maximum-likelihood method.
1997,
Pubmed
Murzin,
SCOP: a structural classification of proteins database for the investigation of sequences and structures.
1995,
Pubmed
Nowotny,
Crystal structures of RNase H bound to an RNA/DNA hybrid: substrate specificity and metal-dependent catalysis.
2005,
Pubmed
Otwinowski,
Processing of X-ray diffraction data collected in oscillation mode.
1997,
Pubmed
Renzi,
Characterization of the sequences encoding for Xenopus laevis box C/D snoRNP Nop56 protein.
2002,
Pubmed
,
Xenbase
Shindyalov,
A database and tools for 3-D protein structure comparison and alignment using the Combinatorial Extension (CE) algorithm.
2001,
Pubmed
Snijder,
Unique and conserved features of genome and proteome of SARS-coronavirus, an early split-off from the coronavirus group 2 lineage.
2003,
Pubmed
Terwilliger,
SOLVE and RESOLVE: automated structure solution and density modification.
2003,
Pubmed
Zegers,
Crystal structure of RNase T1 with 3'-guanylic acid and guanosine.
1994,
Pubmed
Zegers,
The structures of RNase A complexed with 3'-CMP and d(CpA): active site conformation and conserved water molecules.
1994,
Pubmed
