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RNA
2006 Nov 01;1211:1993-2004. doi: 10.1261/rna.125506.
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RNA aptamers binding the double-stranded RNA-binding domain.
Hallegger M
,
Taschner A
,
Jantsch MF
.
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Specific RNA recognition of proteins containing the double-strand RNA-binding domain (dsRBD) is essential for several biological pathways such as ADAR-mediated adenosine deamination, localization of RNAs by Staufen, or RNA cleavage by RNAse III. Structural analysis has demonstrated the lack of base-specific interactions of dsRBDs with either a perfect RNA duplex or an RNA hairpin. We therefore asked whether in vitro selections performed in parallel with individual dsRBDs could yield RNAs that are specifically recognized by the dsRBD on which they were selected . To this end, SELEX experiments were performed using either the second dsRBD of the RNA-editing enzyme ADAR1 or the second dsRBD of Xlrbpa, a homolog of TRBP that is involved in RISC formation. Several RNA families with high binding capacities for dsRBDs were isolated from either SELEX experiment, but no discrimination of these RNAs by different dsRBDs could be detected. The selected RNAs are highly structured, and binding regions map to two neighboring stem-loops that presumably form stacked helices and are interrupted by mismatches and bulges. Despite the lack of selective binding of SELEX RNAs to individual dsRBDS, selected RNAs can efficiently interfere with RNA editing in vivo.
Bass,
RNA editing by adenosine deaminases that act on RNA.
2002, Pubmed
Bass,
RNA editing by adenosine deaminases that act on RNA.
2002,
Pubmed
Bass,
An unwinding activity that covalently modifies its double-stranded RNA substrate.
1988,
Pubmed
,
Xenbase
Bass,
Binding properties of newly identified Xenopus proteins containing dsRNA-binding motifs.
1994,
Pubmed
,
Xenbase
Bevilacqua,
Minor-groove recognition of double-stranded RNA by the double-stranded RNA-binding domain from the RNA-activated protein kinase PKR.
1996,
Pubmed
Bevilacqua,
Binding of the protein kinase PKR to RNAs with secondary structure defects: role of the tandem A-G mismatch and noncontiguous helixes.
1998,
Pubmed
Brooks,
The double-stranded RNA-binding domains of Xenopus laevis ADAR1 exhibit different RNA-binding behaviors.
1998,
Pubmed
,
Xenbase
Burns,
Regulation of serotonin-2C receptor G-protein coupling by RNA editing.
1997,
Pubmed
Bycroft,
NMR solution structure of a dsRNA binding domain from Drosophila staufen protein reveals homology to the N-terminal domain of ribosomal protein S5.
1995,
Pubmed
Chanfreau,
Conservation of RNase III processing pathways and specificity in hemiascomycetes.
2003,
Pubmed
Chanfreau,
Recognition of a conserved class of RNA tetraloops by Saccharomyces cerevisiae RNase III.
2000,
Pubmed
Doyle,
Distinct in vivo roles for double-stranded RNA-binding domains of the Xenopus RNA-editing enzyme ADAR1 in chromosomal targeting.
2003,
Pubmed
,
Xenbase
Eckmann,
Xlrbpa, a double-stranded RNA-binding protein associated with ribosomes and heterogeneous nuclear RNPs.
1997,
Pubmed
,
Xenbase
Higuchi,
Point mutation in an AMPA receptor gene rescues lethality in mice deficient in the RNA-editing enzyme ADAR2.
2000,
Pubmed
Hitti,
The double-stranded RNA-binding protein X1rbpa promotes RNA strand annealing.
1998,
Pubmed
,
Xenbase
Kharrat,
Structure of the dsRNA binding domain of E. coli RNase III.
1995,
Pubmed
Klug,
All you wanted to know about SELEX.
1994,
Pubmed
Kumar,
Nuclear antisense RNA induces extensive adenosine modifications and nuclear retention of target transcripts.
1997,
Pubmed
Levanon,
Evolutionarily conserved human targets of adenosine to inosine RNA editing.
2005,
Pubmed
Liu,
Chimeric double-stranded RNA-specific adenosine deaminase ADAR1 proteins reveal functional selectivity of double-stranded RNA-binding domains from ADAR1 and protein kinase PKR.
2000,
Pubmed
Lomeli,
Control of kinetic properties of AMPA receptor channels by nuclear RNA editing.
1994,
Pubmed
,
Xenbase
Lowman,
On the recognition of helical RNA by cobra venom V1 nuclease.
1986,
Pubmed
Mathews,
Expanded sequence dependence of thermodynamic parameters improves prediction of RNA secondary structure.
1999,
Pubmed
Morse,
RNA hairpins in noncoding regions of human brain and Caenorhabditis elegans mRNA are edited by adenosine deaminases that act on RNA.
2002,
Pubmed
Morse,
Detection of inosine in messenger RNA by inosine-specific cleavage.
1997,
Pubmed
Murphy,
Numerous transitions in human parainfluenza virus 3 RNA recovered from persistently infected cells.
1991,
Pubmed
Ramos,
RNA recognition by a Staufen double-stranded RNA-binding domain.
2000,
Pubmed
Ryter,
Molecular basis of double-stranded RNA-protein interactions: structure of a dsRNA-binding domain complexed with dsRNA.
1998,
Pubmed
,
Xenbase
St Johnston,
A conserved double-stranded RNA-binding domain.
1992,
Pubmed
,
Xenbase
Stefl,
Resonance assignments of the double-stranded RNA-binding domains of adenosine deaminase acting on RNA 2 (ADAR2).
2005,
Pubmed
Stefl,
A novel RNA pentaloop fold involved in targeting ADAR2.
2005,
Pubmed
Stephens,
The binding selectivity of ADAR2's dsRBMs contributes to RNA-editing selectivity.
2004,
Pubmed
Wong,
Generalized and localized biased hypermutation affecting the matrix gene of a measles virus strain that causes subacute sclerosing panencephalitis.
1989,
Pubmed
Wong,
Substrate recognition by ADAR1 and ADAR2.
2001,
Pubmed
Wu,
Structural basis for recognition of the AGNN tetraloop RNA fold by the double-stranded RNA-binding domain of Rnt1p RNase III.
2004,
Pubmed
Zacharias,
Bulge-induced bends in RNA: quantification by transient electric birefringence.
1995,
Pubmed
Zheng,
Activation of the protein kinase PKR by short double-stranded RNAs with single-stranded tails.
2004,
Pubmed
Zuker,
Mfold web server for nucleic acid folding and hybridization prediction.
2003,
Pubmed
