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XB-ART-35201
Biol Cell May 1, 2007; 99 (5): 239-50.

Translational control of maskin mRNA by its 3'' untranslated region.

Meijer HA , Radford HE , Wilson LS , Lissenden S , de Moor CH .


Abstract
BACKGROUND INFORMATION: Maskin is a member of the TACC (transforming acidic coiled-coil) domain proteins found in Xenopus laevis oocytes and embryos. It has been implicated in the co-ordination of the spindle and has been reported to mediate translational repression of cyclin B1 mRNA. RESULTS: In the present study, we report that maskin mRNA is translationally repressed at the level of initiation in stage 4 oocytes and becomes activated in stage 6 oocytes. The translational repression of maskin mRNA correlates with the presence of a short poly(A) tail on this mRNA in stage 4 oocytes. The 3''-UTR (untranslated region) of maskin can confer the translational regulation to a reporter mRNA, and so can the 3''-UTR of human TACC3. A conserved GUCU repeat element was found to repress translation in both stage 4 and stage 6 oocytes, but deletion of this element did not abrogate repression in stage 4 oocytes. UV cross-linking experiments indicated that overlapping sets of proteins bind efficiently to both the maskin and the cyclin B1 3''-UTRs. As reported previously, CPEB [CPE (cytoplasmic polyadenylation element)-binding protein] binds to the cyclin B1 3''-UTR, but its binding to the maskin 3''-UTR is minimal. By RNA affinity chromatography and MS, we identified the EDEN-BP [EDEN (embryonic deadenylation element)-binding protein] as one of the proteins binding to both the maskin and the cyclin B1 3''-UTRs. CONCLUSIONS: Maskin mRNA is translationally regulated by at least two repressor elements and an activation element. One of the repessor elements is the evolutionarily conserved GUCU repeat. EDEN-BP binds to both the maskin and cyclin B1 3''-UTRs, indicating it may be involved in the deadenylation of these mRNAs.

PubMed ID: 17241108
PMC ID: PMC1888485
Article link: Biol Cell
Grant support: [+]

Species referenced: Xenopus laevis
Genes referenced: cpeb1 tacc3

References [+] :
Albee, Phosphorylation of maskin by Aurora-A is regulated by RanGTP and importin beta. 2006, Pubmed, Xenbase