XB-ART-35277Cytogenet Genome Res January 1, 2007; 116 (1-2): 110-2.
Localization of the single copy gene Mdh2 on Xenopus tropicalis chromosomes by FISH-TSA.
A single copy gene, mitochondrial malate dehydrogenase 2 (Mdh2), was localized on Xenopus tropicalis chromosomes by fluorescence in situ hybridization coupled with tyramide signal amplification (FISH-TSA). The respective cDNA was cloned and sequenced. The labeled probe hybridized with a subcentromeric region of the long arms of homologous chromosomes 3. Results of comparison of the gene localization with previously mapped X. laevis paralogs strongly suggest a common evolutionary origin of chromosomes 3 and 8 in X. laevis and chromosome 3 in X. tropicalis. This is the first time that a single copy gene has been visualized on X. tropicalis chromosomes. The FISH-TSA method gives a strong signal with a 1-kb labeled probe.
PubMed ID: 17268187
Article link: Cytogenet Genome Res
Genes referenced: kidins220 mdh2