Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Mol Cell
2006 Nov 03;243:481-7. doi: 10.1016/j.molcel.2006.09.012.
Show Gene links
Show Anatomy links
RSC exploits histone acetylation to abrogate the nucleosomal block to RNA polymerase II elongation.
Carey M
,
Li B
,
Workman JL
.
???displayArticle.abstract???
The coordinated action of histone acetyltransferases (HATs) and ATP-dependent chromatin remodeling enzymes in promoter-dependent transcription initiation represents a paradigm for how epigenetic information regulates gene expression. However, little is known about how such enzymes function during transcription elongation. Here, we investigated the role of RSC, a bromodomain-containing ATPase, in nucleosome transcription in vitro. Purified S. cerevisiae RNA polymerase II (Pol II) arrests at two primary locations on a positioned mononucleosome. RSC stimulates passage of Pol II through these sites. The function of RSC in elongation requires the energy of ATP hydrolysis. Moreover, the SAGA and NuA4 HATs strongly stimulated RSC's effect on elongation. The stimulation correlates closely with acetyl-CoA-dependent recruitment of RSC to nucleosomes. Thus, RSC can recognize acetylated nucleosomes and facilitate passage of Pol II through them. These data support the view that histone modifications regulate accessibility of the coding region to Pol II.
Allard,
NuA4, an essential transcription adaptor/histone H4 acetyltransferase complex containing Esa1p and the ATM-related cofactor Tra1p.
1999, Pubmed
Allard,
NuA4, an essential transcription adaptor/histone H4 acetyltransferase complex containing Esa1p and the ATM-related cofactor Tra1p.
1999,
Pubmed
Belotserkovskaya,
FACT facilitates transcription-dependent nucleosome alteration.
2003,
Pubmed
Brown,
Activator-dependent regulation of transcriptional pausing on nucleosomal templates.
1996,
Pubmed
Cairns,
RSC, an essential, abundant chromatin-remodeling complex.
1996,
Pubmed
Carrozza,
Histone H3 methylation by Set2 directs deacetylation of coding regions by Rpd3S to suppress spurious intragenic transcription.
2005,
Pubmed
Côté,
Stimulation of GAL4 derivative binding to nucleosomal DNA by the yeast SWI/SNF complex.
1994,
Pubmed
Davie,
Genetic interactions between TFIIS and the Swi-Snf chromatin-remodeling complex.
2000,
Pubmed
Guermah,
Synergistic functions of SII and p300 in productive activator-dependent transcription of chromatin templates.
2006,
Pubmed
Hassan,
Histone acetyltransferase complexes stabilize swi/snf binding to promoter nucleosomes.
2001,
Pubmed
Ito,
p300-mediated acetylation facilitates the transfer of histone H2A-H2B dimers from nucleosomes to a histone chaperone.
2000,
Pubmed
Kadesch,
Studies of in vitro transcription by calf thymus RNA polymerase II using a novel duplex DNA template.
1982,
Pubmed
Kasten,
Tandem bromodomains in the chromatin remodeler RSC recognize acetylated histone H3 Lys14.
2004,
Pubmed
Kireeva,
Nucleosome remodeling induced by RNA polymerase II: loss of the H2A/H2B dimer during transcription.
2002,
Pubmed
Kireeva,
Nature of the nucleosomal barrier to RNA polymerase II.
2005,
Pubmed
Kraus,
p300 and estrogen receptor cooperatively activate transcription via differential enhancement of initiation and reinitiation.
1998,
Pubmed
Li,
The Set2 histone methyltransferase functions through the phosphorylated carboxyl-terminal domain of RNA polymerase II.
2003,
Pubmed
Li,
Preferential occupancy of histone variant H2AZ at inactive promoters influences local histone modifications and chromatin remodeling.
2005,
Pubmed
Lieb,
Control of transcription through intragenic patterns of nucleosome composition.
2005,
Pubmed
Liu,
Role of C-terminal domain phosphorylation in RNA polymerase II transcription through the nucleosome.
2003,
Pubmed
Luger,
Crystal structure of the nucleosome core particle at 2.8 A resolution.
1997,
Pubmed
Pavri,
Histone H2B monoubiquitination functions cooperatively with FACT to regulate elongation by RNA polymerase II.
2006,
Pubmed
Peterson,
Promoter targeting and chromatin remodeling by the SWI/SNF complex.
2000,
Pubmed
Reinberg,
de FACTo nucleosome dynamics.
2006,
Pubmed
Roeder,
Transcriptional regulation and the role of diverse coactivators in animal cells.
2005,
Pubmed
Saha,
Chromatin remodeling by RSC involves ATP-dependent DNA translocation.
2002,
Pubmed
Saha,
Chromatin remodeling through directional DNA translocation from an internal nucleosomal site.
2005,
Pubmed
,
Xenbase
Sawadogo,
Energy requirement for specific transcription initiation by the human RNA polymerase II system.
1984,
Pubmed
Shilatifard,
The RNA polymerase II elongation complex.
2003,
Pubmed
Sims,
Elongation by RNA polymerase II: the short and long of it.
2004,
Pubmed
Soutourina,
Rsc4 connects the chromatin remodeler RSC to RNA polymerases.
2006,
Pubmed
Steger,
Purified histone acetyltransferase complexes stimulate HIV-1 transcription from preassembled nucleosomal arrays.
1998,
Pubmed
Studitsky,
Chromatin remodeling by RNA polymerases.
2004,
Pubmed
Thåström,
Nucleosomal locations of dominant DNA sequence motifs for histone-DNA interactions and nucleosome positioning.
2004,
Pubmed
Tran,
The chromo domain protein chd1p from budding yeast is an ATP-dependent chromatin-modifying factor.
2000,
Pubmed
Tsukiyama,
ISWI, a member of the SWI2/SNF2 ATPase family, encodes the 140 kDa subunit of the nucleosome remodeling factor.
1995,
Pubmed
Workman,
Nucleosome displacement in transcription.
2006,
Pubmed
