XB-ART-35573Nat Methods April 1, 2007; 4 (4): 319-21.
Subunit counting in membrane-bound proteins.
The subunit number and stoichiometry of membrane-bound proteins are difficult to determine without disrupting their membrane environment. Here we describe a single-molecule technique for counting subunits of proteins in live cell membranes by observing bleaching steps of GFP fused to a protein of interest. After testing the method with proteins of known stoichiometry expressed in Xenopus laevis oocytes, we resolved the composition of NMDA receptors composed of NR1 and NR3 subunits.
PubMed ID: 17369835
PMC ID: PMC2744285
Article link: Nat Methods
Grant support: R01 NS035549-11 NINDS NIH HHS , R01 NS035549-12 NINDS NIH HHS , R01 NS035549 NINDS NIH HHS
Genes referenced: grin1 nodal3.1