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Dev Dyn June 1, 2007; 236 (6): 1633-49.

Xenopus cDNA microarray identification of genes with endodermal organ expression.

The endoderm is classically defined as the innermost layer of three Metazoan germ layers. During organogenesis, the endoderm gives rise to the digestive and respiratory tracts as well as associated organs such as the liver, pancreas, and lung. At present, however, how the endoderm forms the variety of cell types of digestive and respiratory tracts as well as the budding organs is not well understood. In order to investigate the molecular basis and mechanism of organogenesis and to identify the endodermal organ-related marker genes, we carried out microarray analysis using Xenopus cDNA chips. To achieve this goal, we isolated the Xenopus gut endoderm from three different stages of Xenopus organogenesis, and separated each stage of gut endoderm into anterior and posterior regions. Competitive hybridization of cDNA between the anterior and posterior endoderm regions, to screen genes that specifically expressed in the major organs, revealed 915 candidates. We then selected 104 clones for in situ hybridization analysis. Here, we report the identification and expression patterns of the 104 Xenopus endodermal genes, which would serve as useful markers for studying endodermal organ development.

PubMed ID: 17474120
Article link: Dev Dyn

Genes referenced: odc1 tubb sox2 dstn tspan1 cd82 oxct1 sephs1 crebrf cfap52 c8b ndufaf3 msn rfx3 stard10 cnfn.1 ag1 nom1 hdac1 fzd7 etv1 id4 sema6d ddx3x pfkm iars desi2 serpinb10 col4a5 meis1 ca2 gata6 sec61b serbp1 cldn4 kng1 atp1b3 tbx2 entpd2 mafb a2m hrg xbp1 cers3 zbtb46 nr2f2 ift88 sfrp1 rab3d anxa1.1 epcam myc bmpr1a fst hes4 slc6a14.2 rpl6 hspd1 btf3 hmgn2 hmgn1 usf1 apoe jund gpd2 cers2 impdh2

GEO Series:
   GSE7129: NCBI

Article Images: [+] show captions

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