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XB-ART-35966
Dev Biol. July 1, 2007; 307 (1): 152-64.

Xenopus Bicaudal-C is required for the differentiation of the amphibian pronephros.

Tran U , Pickney LM , Ozpolat BD , Wessely O .


Abstract
The RNA-binding molecule Bicaudal-C regulates embryonic development in Drosophila and Xenopus. Interestingly, mouse mutants of Bicaudal-C do not show early patterning defects, but instead develop polycystic kidney disease (PKD). To further investigate the molecular mechanism of Bicaudal-C in kidney development, we analyzed its function in the developing amphibian pronephros. Bicaudal-C mRNA was present in the epithelial structures of the Xenopus pronephros, the tubules and the duct, but not the glomus. Inhibition of the translation of endogenous Bicaudal-C with antisense morpholino oligomers (xBic-C-MO) led to a PKD-like phenotype in Xenopus. Embryos lacking Bicaudal-C developed generalized edemas and dilated pronephric tubules and ducts. This phenotype was caused by impaired differentiation of the pronephros. Molecular markers specifically expressed in the late distal tubule were absent in xBic-C-MO-injected embryos. Furthermore, Bicaudal-C was not required for primary cilia formation, an important organelle affected in PKD. These data support the idea that Bicaudal-C functions downstream or parallel of a cilia-regulated signaling pathway. This pathway is required for terminal differentiation of the late distal tubule of the Xenopus pronephros and regulates renal epithelial cell differentiation, which--when disrupted--results in PKD.

PubMed ID: 17521625
PMC ID: PMC1976305
Article link: Dev Biol.
Grant support: 5R21DK070671-02 NIDDK NIH HHS , R21 DK070671-01 NIDDK NIH HHS , R21 DK070671-02 NIDDK NIH HHS , R21 DK070671 NIDDK NIH HHS , R21 DK070671 NIDDK NIH HHS , R21 DK070671-01 NIDDK NIH HHS , R21 DK070671-02 NIDDK NIH HHS , 5R21DK070671-02 NIDDK NIH HHS

Genes referenced: atp1b1 bicc1 ca2 clcnkb dnah9 foxj1.2 gata3 hnf1b ift88 kcnj1 lhx1 nphs1 pax2 pax8 prkd1 slc12a1 slc12a3 slc4a4 slc5a1.2 tuba4b wt1

Antibodies referenced: Tuba4b Ab4
Morpholinos referenced: bicc1 MO1 bicc1 MO2 ift88 MO1

References:
Agius, 2000, Pubmed, Xenbase[+]


Article Images: [+] show captions

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