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XB-ART-35968
BMC Dev Biol May 24, 2007; 7 56.

Regeneration of neural crest derivatives in the Xenopus tadpole tail.



Abstract
BACKGROUND: After amputation of the Xenopus tadpole tail, a functionally competent new tail is regenerated. It contains spinal cord, notochord and muscle, each of which has previously been shown to derive from the corresponding tissue in the stump. The regeneration of the neural crest derivatives has not previously been examined and is described in this paper. RESULTS: Labelling of the spinal cord by electroporation, or by orthotopic grafting of transgenic tissue expressing GFP, shows that no cells emigrate from the spinal cord in the course of regeneration. There is very limited regeneration of the spinal ganglia, but new neurons as well as fibre tracts do appear in the regenerated spinal cord and the regenerated tail also contains abundant peripheral innervation.The regenerated tail contains a normal density of melanophores. Cell labelling experiments show that melanophores do not arise from the spinal cord during regeneration, nor from the mesenchymal tissues of the skin, but they do arise by activation and proliferation of pre-existing melanophore precursors. If tails are prepared lacking melanophores, then the regenerates also lack them. CONCLUSION: On regeneration there is no induction of a new neural crest similar to that seen in embryonic development. However there is some regeneration of neural crest derivatives. Abundant melanophores are regenerated from unpigmented precursors, and, although spinal ganglia are not regenerated, sufficient sensory systems are produced to enable essential functions to continue.

PubMed ID: 17521450
PMC ID: PMC1890292
Article link: BMC Dev Biol
Grant support: [+]
Genes referenced: dct kit mitf ngfr pou4f1
Antibodies: Elavl3 Ab1 Isl1/2 Ab1 Kit Ab1 Nefh Ab1 Tubb3 Ab1


Article Images: [+] show captions
References [+] :
An, Differentiation and maturation of zebrafish dorsal root and sympathetic ganglion neurons. 2002, Pubmed


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