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XB-ART-35984
Science. May 18, 2007; 316 (5827): 1043-6.

Wilms tumor suppressor WTX negatively regulates WNT/beta-catenin signaling.

Major MB , Camp ND , Berndt JD , Yi X , Goldenberg SJ , Hubbert C , Biechele TL , Gingras AC , Zheng N , Maccoss MJ , Angers S , Moon RT .


Abstract
Aberrant WNT signal transduction is involved in many diseases. In colorectal cancer and melanoma, mutational disruption of proteins involved in the degradation of beta-catenin, the key effector of the WNT signaling pathway, results in stabilization of beta-catenin and, in turn, activation of transcription. We have used tandem-affinity protein purification and mass spectrometry to define the protein interaction network of the beta-catenin destruction complex. This assay revealed that WTX, a protein encoded by a gene mutated in Wilms tumors, forms a complex with beta-catenin, AXIN1, beta-TrCP2 (beta-transducin repeat-containing protein 2), and APC (adenomatous polyposis coli). Functional analyses in cultured cells, Xenopus, and zebrafish demonstrate that WTX promotes beta-catenin ubiquitination and degradation, which antagonize WNT/beta-catenin signaling. These data provide a possible mechanistic explanation for the tumor suppressor activity of WTX.

PubMed ID: 17510365
Article link: Science.

Genes referenced: axin1
Antibodies referenced:

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