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XB-ART-36275
Dev Cell August 1, 2007; 13 (2): 226-41.

The secreted serine protease xHtrA1 stimulates long-range FGF signaling in the early Xenopus embryo.

Hou S , Maccarana M , Min TH , Strate I , Pera EM .


Abstract
We found that the secreted serine protease xHtrA1, expressed in the early embryo and transcriptionally activated by FGF signals, promotes posterior development in mRNA-injected Xenopus embryos. xHtrA1 mRNA led to the induction of secondary tail-like structures, expansion of mesoderm, and formation of ectopic neurons in an FGF-dependent manner. An antisense morpholino oligonucleotide or a neutralizing antibody against xHtrA1 had the opposite effects. xHtrA1 activates FGF/ERK signaling and the transcription of FGF genes. We show that Xenopus Biglycan, Syndecan-4, and Glypican-4 are proteolytic targets of xHtrA1 and that heparan sulfate and dermatan sulfate trigger posteriorization, mesoderm induction, and neuronal differentiation via the FGF signaling pathway. The results are consistent with a mechanism by which xHtrA1, through cleaving proteoglycans, releases cell-surface-bound FGF ligands and stimulates long-range FGF signaling.

PubMed ID: 17681134
Article link: Dev Cell

Genes referenced: bgn bmp4 egr2 en2 fgf4 fgf8 foxg1 gpc1 gpc4 h4c4 hspa9 htra1 ins krt12.4 mapk1 myod1 nkx2-5 nppa otx2 prss1 rax sdc4 shh snai2 sox2 szl tbxt tubb2b
GO keywords: fibroblast growth factor receptor signaling pathway [+]
Morpholinos: htra1 MO1


Article Images: [+] show captions
References:
Gallagher, 2007, Pubmed, Xenbase


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