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XB-ART-36480
J Cell Biol July 2, 2007; 178 (1): 43-56.

A role for Rab5 in structuring the endoplasmic reticulum.

Audhya A , Desai A , Oegema K .


Abstract
The endoplasmic reticulum (ER) is a contiguous network of interconnected membrane sheets and tubules. The ER is differentiated into distinct domains, including the peripheral ER and nuclear envelope. Inhibition of two ER proteins, Rtn4a and DP1/NogoA, was previously shown to inhibit the formation of ER tubules in vitro. We show that the formation of ER tubules in vitro also requires a Rab family GTPase. Characterization of the 29 Caenorhabditis elegans Rab GTPases reveals that depletion of RAB-5 phenocopies the defects in peripheral ER structure that result from depletion of RET-1 and YOP-1, the C. elegans homologues of Rtn4a and DP1/NogoA. Perturbation of endocytosis by other means did not affect ER structure; the role of RAB-5 in ER morphology is thus independent of its well-studied requirement for endocytosis. RAB-5 and YOP-1/RET-1 also control the kinetics of nuclear envelope disassembly, which suggests an important role for the morphology of the peripheral ER in this process.

PubMed ID: 17591921
PMC ID: PMC2064419
Article link: J Cell Biol

Genes referenced: emb rab5a ret rho rho.2 sult1e1 tbx2 tfdp1


Article Images: [+] show captions
References [+] :
Audhya, A complex containing the Sm protein CAR-1 and the RNA helicase CGH-1 is required for embryonic cytokinesis in Caenorhabditis elegans. 2005, Pubmed


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