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XB-ART-36493
Dev Biol. November 15, 2007; 311 (2): 623-35.

Redundancy and evolution of GATA factor requirements in development of the myocardium.

Peterkin T , Gibson A , Patient R .


Abstract
The transcription factors, GATA4, 5 and 6, recognize the same DNA sequence and are all expressed in the developing myocardium. However, knockout studies in the mouse have indicated that none of them are absolutely required for the specification of the myocardium. Here we present evidence for redundancy in this family for the first time. Using morpholinos in both Xenopus and zebrafish embryos, we show that GATA4 knockdown, for example, only affects cardiac marker expression in the absence of either GATA5 or GATA6. A similar situation pertains for GATA5 in Xenopus whereas, in zebrafish, GATA5 (faust) plays a major role in driving the myocardial programme. This requirement for GATA5 in zebrafish is for induction of the myocardium, in contrast to the GATA6 requirement in both species, which is for differentiation. This early role for GATA5 in zebrafish correlates with its earlier expression and with an earlier requirement for BMP signalling, suggesting that a mutual maintenance loop for GATA, BMP and Nkx expression is the evolutionarily conserved entity.

PubMed ID: 17869240
PMC ID: PMC2279743
Article link: Dev Biol.
Grant support: MC_U137981013 Medical Research Council , MRC_MC_U137981013 Medical Research Council , MC_U137981013 Medical Research Council

Genes referenced: gata4 gata5 gata6 hand1 mlc1 myh15 nkx2-3 nkx2-5 tbx5 actc1

Morpholinos referenced: gata4 MO2 gata4 MO3 gata5 MO3

References:
Afouda, 2005, Pubmed, Xenbase[+]


Article Images: [+] show captions

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