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XB-ART-36566
Nat Methods September 1, 2007; 4 (9): 741-6.

Bright far-red fluorescent protein for whole-body imaging.

Shcherbo D , Merzlyak EM , Chepurnykh TV , Fradkov AF , Ermakova GV , Solovieva EA , Lukyanov KA , Bogdanova EA , Zaraisky AG , Lukyanov S , Chudakov DM .


Abstract
For deep imaging of animal tissues, the optical window favorable for light penetration is in near-infrared wavelengths, which requires proteins with emission spectra in the far-red wavelengths. Here we report a far-red fluorescent protein, named Katushka, which is seven- to tenfold brighter compared to the spectrally close HcRed or mPlum, and is characterized by fast maturation as well as a high pH-stability and photostability. These unique characteristics make Katushka the protein of choice for visualization in living tissues. We demonstrate superiority of Katushka for whole-body imaging by direct comparison with other red and far-red fluorescent proteins. We also describe a monomeric version of Katushka, named mKate, which is characterized by high brightness and photostability, and should be an excellent fluorescent label for protein tagging in the far-red part of the spectrum.

PubMed ID: 17721542
Article link: Nat Methods
Grant support: [+]