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XB-ART-37197
Dev Biol March 1, 2008; 315 (1): 203-16.

TGF-beta signaling is required for multiple processes during Xenopus tail regeneration.

Ho DM , Whitman M .


Abstract
Xenopus tadpoles can fully regenerate all major tissue types following tail amputation. TGF-beta signaling plays essential roles in growth, repair, specification, and differentiation of tissues throughout development and adulthood. We examined the localization of key components of the TGF-beta signaling pathway during regeneration and characterized the effects of loss of TGF-beta signaling on multiple regenerative events. Phosphorylated Smad2 (p-Smad2) is initially restricted to the p63+ basal layer of the regenerative epithelium shortly after amputation, and is later found in multiple tissue types in the regeneration bud. TGF-beta ligands are also upregulated throughout regeneration. Treatment of amputated tails with SB-431542, a specific and reversible inhibitor of TGF-beta signaling, blocks tail regeneration at multiple points. Inhibition of TGF-beta signaling immediately following tail amputation reversibly prevents formation of a wound epithelium over the future regeneration bud. Even brief inhibition immediately following amputation is sufficient, however, to irreversibly block the establishment of structures and cell types that characterize regenerating tissue and to prevent the proper activation of BMP and ERK signaling pathways. Inhibition of TGF-beta signaling after regeneration has already commenced blocks cell proliferation in the regeneration bud. These data reveal several spatially and temporally distinct roles for TGF-beta signaling during regeneration: (1) wound epithelium formation, (2) establishment of regeneration bud structures and signaling cascades, and (3) regulation of cell proliferation.

PubMed ID: 18234181
PMC ID: PMC2292344
Article link: Dev Biol
Grant support: [+]
Genes referenced: en1 hpse mapk1 msx1 nhs pax7 smad1 smad2 tgfb1 tgfb2 tp63
GO keywords: regeneration [+]
Antibodies: Notochord Ab1


Article Images: [+] show captions
References [+] :
Arany, Smad3 deficiency alters key structural elements of the extracellular matrix and mechanotransduction of wound closure. 2006, Pubmed


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