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Dorsal-ventral gradient for neuronal plasticity in the embryonic spinal cord.
Pineda RH
,
Ribera AB
.
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Within the developing Xenopus spinal cord, voltage-gated potassium (Kv) channel genes display different expression patterns, many of which occur in opposing dorsal-ventral gradients. Regional differences in Kv gene expression would predict different patterns of potassium current (I(Kv)) regulation. However, during the first 24 h of postmitotic differentiation, all primary spinal neurons undergo a temporally coordinated upregulation of I(Kv) density that shortens the duration of the action potential. Here, we tested whether spinal neurons demonstrate regional differences in I(Kv) regulation subsequent to action potential maturation. We show that two types of neurons, I and II, can be identified in culture on the basis of biophysical and pharmacological properties of I(Kv) and different firing patterns. Chronic increases in extracellular potassium, a signature of high neuronal activity, do not alter excitability properties of either neuron type. However, elevating extracellular potassium acutely after the period of action potential maturation leads to different changes in membrane properties of the two types of neurons. I(Kv) of type I neurons gains sensitivity to the blocker XE991, whereas type II neurons increase I(Kv) density and fire fewer action potentials. Moreover, by recording from neurons in vivo, we found that primary spinal neurons can be identified as either type I or type II. Type I neurons predominate in dorsal regions, whereas type II neurons localize to ventral regions. The findings reveal a dorsal-ventral gradient for I(Kv) regulation and a novel form of neuronal plasticity in spinal cord neurons.
Baccaglini,
Developmental changes in the inward current of the action potential of Rohon-Beard neurones.
1977, Pubmed,
Xenbase
Baccaglini,
Developmental changes in the inward current of the action potential of Rohon-Beard neurones.
1977,
Pubmed
,
Xenbase
Barish,
Differentiation of voltage-gated potassium current and modulation of excitability in cultured amphibian spinal neurones.
1986,
Pubmed
Bezanilla,
Inactivation of the sodium channel. I. Sodium current experiments.
1977,
Pubmed
Bixby,
The appearance and development of neurotransmitter sensitivity in Xenopus embryonic spinal neurones in vitro.
1984,
Pubmed
,
Xenbase
Blaine,
Carboxyl tail region of the Kv2.2 subunit mediates novel developmental regulation of channel density.
2004,
Pubmed
,
Xenbase
Blair,
The timing of protein synthesis required for the development of the sodium action potential in embryonic spinal neurons.
1983,
Pubmed
,
Xenbase
Burger,
Xenopus spinal neurons express Kv2 potassium channel transcripts during embryonic development.
1996,
Pubmed
,
Xenbase
Castellano,
Identification and functional characterization of a K+ channel alpha-subunit with regulatory properties specific to brain.
1997,
Pubmed
Chitnis,
Primary neurogenesis in Xenopus embryos regulated by a homologue of the Drosophila neurogenic gene Delta.
1995,
Pubmed
,
Xenbase
Cooper,
M-channels: neurological diseases, neuromodulation, and drug development.
2003,
Pubmed
Dale,
A large, sustained Na(+)- and voltage-dependent K+ current in spinal neurons of the frog embryo.
1993,
Pubmed
,
Xenbase
Davis,
Maintaining the stability of neural function: a homeostatic hypothesis.
2001,
Pubmed
Desarmenien,
Role of calcium and protein kinase C in development of the delayed rectifier potassium current in Xenopus spinal neurons.
1991,
Pubmed
,
Xenbase
Desarmenien,
In vivo development of voltage-dependent ionic currents in embryonic Xenopus spinal neurons.
1993,
Pubmed
,
Xenbase
Fantozzi,
Bone morphogenetic protein-2 upregulates expression and function of voltage-gated K+ channels in human pulmonary artery smooth muscle cells.
2006,
Pubmed
Fry,
Cloning and expression of three K+ channel cDNAs from Xenopus muscle.
2001,
Pubmed
,
Xenbase
Fry,
Properties of Xenopus Kv1.10 channels expressed in HEK293 cells.
2004,
Pubmed
,
Xenbase
Golowasch,
Activity-dependent regulation of potassium currents in an identified neuron of the stomatogastric ganglion of the crab Cancer borealis.
1999,
Pubmed
Gribkoff,
The therapeutic potential of neuronal KCNQ channel modulators.
2003,
Pubmed
,
Xenbase
Gurantz,
Temporal regulation of Shaker- and Shab-like potassium channel gene expression in single embryonic spinal neurons during K+ current development.
1996,
Pubmed
,
Xenbase
Gurantz,
Sustained upregulation in embryonic spinal neurons of a Kv3.1 potassium channel gene encoding a delayed rectifier current.
2000,
Pubmed
,
Xenbase
Hamill,
Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches.
1981,
Pubmed
Harris,
Neuronal determination without cell division in Xenopus embryos.
1991,
Pubmed
,
Xenbase
Harris,
Hydrophobic mutations alter the movement of Mg2+ in the pore of voltage-gated potassium channels.
1996,
Pubmed
,
Xenbase
JONES,
The culture of small aggregates of amphibian embryonic cells in vitro.
1963,
Pubmed
Jones,
Overexpression of a potassium channel gene perturbs neural differentiation.
1994,
Pubmed
,
Xenbase
Kerschensteiner,
Heteromeric assembly of Kv2.1 with Kv9.3: effect on the state dependence of inactivation.
1999,
Pubmed
,
Xenbase
Lazaroff,
Xenopus embryonic spinal neurons express potassium channel Kvbeta subunits.
1999,
Pubmed
,
Xenbase
Lopatin,
Internal Na+ and Mg2+ blockade of DRK1 (Kv2.1) potassium channels expressed in Xenopus oocytes. Inward rectification of a delayed rectifier.
1994,
Pubmed
,
Xenbase
McLean,
A topographic map of recruitment in spinal cord.
2007,
Pubmed
O'Dowd,
Development of voltage-dependent calcium, sodium, and potassium currents in Xenopus spinal neurons.
1988,
Pubmed
,
Xenbase
Olsen,
Differential distribution of Kir4.1 in spinal cord astrocytes suggests regional differences in K+ homeostasis.
2007,
Pubmed
Ottschytsch,
Domain analysis of Kv6.3, an electrically silent channel.
2005,
Pubmed
Patel,
Kv2.1/Kv9.3, a novel ATP-dependent delayed-rectifier K+ channel in oxygen-sensitive pulmonary artery myocytes.
1997,
Pubmed
Pineda,
Developmental, molecular, and genetic dissection of INa in vivo in embryonic zebrafish sensory neurons.
2005,
Pubmed
Post,
Kv2.1 and electrically silent Kv6.1 potassium channel subunits combine and express a novel current.
1996,
Pubmed
,
Xenbase
Ribera,
A critical period of transcription required for differentiation of the action potential of spinal neurons.
1989,
Pubmed
,
Xenbase
Ribera,
Primary sensory neurons express a Shaker-like potassium channel gene.
1993,
Pubmed
,
Xenbase
Ribera,
Homogeneous development of electrical excitability via heterogeneous ion channel expression.
1996,
Pubmed
,
Xenbase
Ribera,
Zebrafish touch-insensitive mutants reveal an essential role for the developmental regulation of sodium current.
1998,
Pubmed
Robbins,
KCNQ potassium channels: physiology, pathophysiology, and pharmacology.
2001,
Pubmed
Salinas,
New modulatory alpha subunits for mammalian Shab K+ channels.
1997,
Pubmed
Sanguinetti,
Coassembly of K(V)LQT1 and minK (IsK) proteins to form cardiac I(Ks) potassium channel.
1996,
Pubmed
,
Xenbase
Spitzer,
Development of the action potential in embryo amphibian neurons in vivo.
1976,
Pubmed
,
Xenbase
Spitzer,
The ionic basis of the resting potential and a slow depolarizing response in Rohon-Beard neurones of Xenopus tadpoles.
1976,
Pubmed
,
Xenbase
Spitzer,
The development of the action potential mechanism of amphibian neurons isolated in culture.
1976,
Pubmed
,
Xenbase
Stocker,
Subunit assembly and domain analysis of electrically silent K+ channel alpha-subunits of the rat Kv9 subfamily.
1999,
Pubmed
,
Xenbase
Tammaro,
Modulation of the voltage-dependent K+ current by intracellular Mg2+ in rat aortic smooth muscle cells.
2005,
Pubmed
Tammaro,
Effects of intracellular magnesium on Kv1.5 and Kv2.1 potassium channels.
2005,
Pubmed
,
Xenbase
Turrigiano,
Activity-dependent changes in the intrinsic properties of cultured neurons.
1994,
Pubmed
Turrigiano,
Homeostatic plasticity in neuronal networks: the more things change, the more they stay the same.
1999,
Pubmed
Vega-Saenz de Miera,
Modification of Kv2.1 K+ currents by the silent Kv10 subunits.
2004,
Pubmed
,
Xenbase
Watt,
Specific frequencies of spontaneous Ca2+ transients upregulate GAD 67 transcripts in embryonic spinal neurons.
2000,
Pubmed
,
Xenbase
Zhu,
Structural and functional characterization of Kv6.2 a new gamma-subunit of voltage-gated potassium channel.
1999,
Pubmed
