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XB-ART-37581
Dev Cell April 1, 2008; 14 (4): 616-23.

Vertebrate CASTOR is required for differentiation of cardiac precursor cells at the ventral midline.



Abstract
The CASTOR (CST) transcription factor was initially identified for its role in maintaining stem cell competence in the Drosophila dorsal midline. Here we report that Xenopus CST affects cardiogenesis. In CST-depleted embryos, cardiomyocytes at the ventral midline arrest and are maintained as cardiac progenitors, while cells in more dorsal regions of the heart undergo their normal program of differentiation. Cardia bifida results from failed midline differentiation, even though cardiac cell migration and initial cell fate specification occur normally. Our fate mapping studies reveal that this ventral midline population of cardiomyocytes ultimately gives rise to the outer curvature of the heart; however, CST-depleted midline cells overproliferate and remain a coherent population of nonintegrated cells positioned on the outer wall of the ventricle. These midline-specific requirements for CST suggest the regulation of cardiomyocyte differentiation is regionalized along a dorsal-ventral axis and that this patterning occurs prior to heart tube formation.

PubMed ID: 18410736
PMC ID: PMC2807748
Article link: Dev Cell
Grant support: [+]
Genes referenced: a2m actc1 actl6a casz1 darmin gata4 gata5 gata6 h3-3a hcst myh4 myh6 nkx2-5 slc5a8.1 sox2 tbx20 tbx5 tnni3 tpm1
Antibodies: Myh6 Ab1 Tpm1 Ab1
Morpholinos: casz1 MO1 casz1 MO2 casz1 MO3


Article Images: [+] show captions
References [+] :
Abu-Issa, Heart field: from mesoderm to heart tube. 2007, Pubmed


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