XB-ART-37630Genesis November 1, 2007; 45 (11): 667-78.
Small heat shock protein Hsp27 is required for proper heart tube formation.
The small heat shock protein Hsp27 has been shown to be involved in a diverse array of cellular processes, including cellular stress response, protein chaperone activity, regulation of cellular glutathione levels, apoptotic signaling, and regulation of actin polymerization and stability. Furthermore, mutation within Hsp27 has been associated with the human congenital neuropathy Charcot-Marie Tooth (CMT) disease. Hsp27 is known to be expressed in developing embryonic tissues; however, little has been done to determine the endogenous requirement for Hsp27 in developing embryos. In this study, we show that depletion of XHSP27 protein results in a failure of cardiac progenitor fusion resulting in cardia bifida. Furthermore, we demonstrate a concomitant disorganization of actin filament organization and defects in myofibril assembly. Moreover, these defects are not associated with alterations in specification or differentiation. We have thus demonstrated a critical requirement for XHSP27 in developing cardiac and skeletal muscle tissues.
PubMed ID: 17987658
PMC ID: PMC2668208
Article link: Genesis
Grant support: R01 HL075256 NHLBI NIH HHS , R01 HL075256-03 NHLBI NIH HHS , R21 HL083965 NHLBI NIH HHS , R21 HL083965-01A1 NHLBI NIH HHS , T32HL69768 NHLBI NIH HHS , T32 HL069768 NHLBI NIH HHS
Genes referenced: actl6a hspb1
Article Images: [+] show captions
|FIG. 2. XHsp27 is expressed in the gastrula, and developing skeletal and cardiac muscle. Whole mount in situ hybridization of X. laevis embryos using an antisense probe specific for XHsp27 at the indicated stages. (a) Dorsal is to the top. (b�i) Anterior is to the left. (j�m) Transverse sections through somitic (j, k) and cardiac (l, m) regions. Dorsal is to the top. b, body muscle; br, brain; h, heart; j, jaw muscle, m, myotome; mc, myocardium.|