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XB-ART-37709
Mech Dev July 1, 2008; 125 (7): 571-86.

A functional screen for genes involved in Xenopus pronephros development.



Abstract
In Xenopus, the pronephros is the functional larval kidney and consists of two identifiable components; the glomus, the pronephric tubules, which can be divided into four separate segments, based on marker gene expression. The simplicity of this organ, coupled with the fact that it displays the same basic organization and function as more complex mesonephros and metanephros, makes this an attractive model to study vertebrate kidney formation. In this study, we have performed a functional screen specifically to identify genes involved in pronephros development in Xenopus. Gain-of-function screens are performed by injecting mRNA pools made from a non-redundant X. tropicalis full-length plasmid cDNA library into X. laevis eggs, followed by sib-selection to identify the single clone that caused abnormal phenotypes in the pronephros. Out of 768 egg and gastrula stage cDNA clones, 31 genes, approximately 4% of the screened clones, affected pronephric marker expression examined by whole mount in situ hybridization or antibody staining. Most of the positive clones had clear expression patterns in pronephros and predicted/established functions highly likely to be involved in developmental processes. In order to carry out a more detailed study, we selected Sox7, Cpeb3, P53csv, Mecr and Dnajc15, which had highly specific expression patterns in the pronephric region. The over-expression of these five selected clones indicated that they caused pronephric abnormalities with different temporal and spatial effects. These results suggest that our strategy to identify novel genes involved in pronephros development was highly successful, and that this strategy is effective for the identification of novel genes involved in late developmental events.

PubMed ID: 18472403
Article link: Mech Dev
Grant support: [+]
Genes referenced: ahctf1 alg10 cops2 cops8 cpeb3 csnk1g1 csnk2b deaf1 dnaja2 dnajc15 dusp11 dusp4 ebag9 elavl2 fubp1 gadd45g gal.2 gnl3 hsf3 kiaa0586 lhx1 lrr1 mall mecr pax8 pcmtd1 phf20l1 snrpd1 snrpd2 sox13 sox3 sox7 spaca5 srd5a1 srsf3 srsf5 supt6h tp53 triap1 vwa8 vwf wdr73 wt1 xkr9
Antibodies: Kidney Ab1 Kidney Ab2


Article Images: [+] show captions


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