Dagenais SL et al. (2004), Foxc2 is expressed in developing lymphatic vess...

XB-ART-37833

Gene Expr Patterns 2004 Oct 01;46:611-9. doi: 10.1016/j.modgep.2004.07.004.

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Foxc2 is expressed in developing lymphatic vessels and other tissues associated with lymphedema-distichiasis syndrome.

Dagenais SL , Hartsough RL , Erickson RP , Witte MH , Butler MG , Glover TW .

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The molecular events involved in lymphatic development are poorly understood. Hence, the genes responsible for hereditary lymphedema are of great interest due to the potential for providing insights into the mechanisms of lymphatic development, the diagnosis, prevention and treatment of lymphedema, and lymphangiogenesis during tumor growth. Mutations in the FOXC2 transcription factor cause a major form of hereditary lymphedema, the lymphedema-distichiasis syndrome. We have conducted a study of Foxc2 expression during mouse development using immunohistochemistry, and examined its expression in lymphatics compared to its paralog Foxc1 and to Vegfr-3, Prox1 and other lymphatic and blood vascular proteins. We have found that Foxc2 is expressed in lymphatic primordia, jugular lymph sacs, lymphatic collectors and capillaries, as well as in podocytes, developing eyelids and other tissues associated with abnormalities in lymphedema-distichiasis syndrome.

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Species referenced: Xenopus
Genes referenced: flt4 foxc1 foxc2 npat nphs1 prox1 sacs slc7a5 tcf3

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	Fig. 4. Foxc2 is expressed in lymphatic vessels throughout the embryo after E14.5. Arrows highlight the lymphatic vessels. Green signal illustrates protein expression. Expression in lymphatic collectors (mesentery) and lymphatic capillaries (skin) are shown at four different developmental ages: E16.5, E18.5, day 1 neonate (D1) and adult (Ad).
	Fig. 5. Lymphatic development in Foxc2 (−/−) nullizygous mice. Asterisks highlight the jugulo-axillary lymph sacs in E12.5 wild-type (WT+/+) and nullizygous (−/−) embryos. Green signal shows expression of the protein in each panel. In panel (h), dilations of submucosal lymphatics in an E18.5 null are demonstrated (arrows). A wildtype control littermate presented in panel (g) shows no dilation.
	Fig. 6. Foxc2 is expressed in additional tissues associated with lymphedema–distichiasis syndrome. Green signal shows Foxc2 protein expression in each panel. (a) In an E18.5 kidney, Foxc2 is expressed in the nuclei of podocytes, which were identified with an antibody to Nephrin (red). (b) Arrows highlight Foxc2 expression in the endothelium of the developing eyelid and the arrowhead shows expression in the periocular mesenchyme of an E15.5 embryo. (c) Foxc2 expression in brown fat is cytoplasmic rather nuclear.