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XB-ART-37867
Dev Dyn July 1, 2008; 237 (7): 1755-66.

Expression cloning in Xenopus identifies RNA-binding proteins as regulators of embryogenesis and Rbmx as necessary for neural and muscle development.

Dichmann DS , Fletcher RB , Harland RM .


Abstract
We have performed an expression cloning screen in Xenopus laevis with the aim of isolating novel gene activities from the neural plate. Of 8,064 clones screened, we isolated 61 clones that affected either neural plate patterning or tadpole morphology. Of these, 20 clones encoded RNA binding proteins, and the majority of these are heterogeneous nuclear ribonucleoproteins (hnRNPs) or SR-proteins, which are associated with alternative splicing. All of these genes are expressed in the nervous system, and in several cases specific to neural tissue. Injecting mRNA encoding these proteins results in neural plate mispatterning and abnormal muscle segmentation. To initiate characterization of these proteins, we selected Rbmx as a candidate for deeper analysis. Using morpholino mediated knockdown, we show that Rbmx is necessary for normal anterior neural plate patterning, neurogenesis, neural crest development, and muscle segmentation.

PubMed ID: 18521943
PMC ID: PMC2562419
Article link: Dev Dyn
Grant support: [+]
Genes referenced: crx en1 gal.2 hnrnpa1 hnrnpc hnrnpl khdrbs1 lbx1 lhx1 myod1 nog pax2 pax6 rax rbm4 rbm4b rbmx snai2 sox2 srsf12 srsf3 tbxt tubb2b
Antibodies: Somite Ab1
Morpholinos: rbmx MO1


Article Images: [+] show captions
References [+] :
Berleth, The role of localization of bicoid RNA in organizing the anterior pattern of the Drosophila embryo. 1988, Pubmed


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