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Cell May 30, 2008; 133 (5): 878-90.

Ectodermal factor restricts mesoderm differentiation by inhibiting p53.

Sasai N , Yakura R , Kamiya D , Nakazawa Y , Sasai Y .

During gastrulation of the amphibian embryo, specification of the three germ layers, endo-, ecto-, and mesoderm, is regulated by maternal and zygotic mechanisms. Although it is known that mesoderm specification requires the cooperation between TGF-beta signaling and p53 activity and requires maternal factors, essential zygotic factors have been elusive. Here, we report that the Zn-finger protein XFDL156 is an ectodermal, zygotic factor that suppresses mesodermal differentiation. XFDL156 overexpression suppresses mesodermal markers, and its depletion induces aberrant mesodermal differentiation in the presumptive ectoderm. Furthermore, we find that XFDL156 and its mammalian homologs interact with the C-terminal regulatory region of p53, thereby inhibiting p53 target gene induction and mesodermal differentiation. Thus, XFDL156 actively restricts mesodermal differentiation in the presumptive ectoderm by controlling the spatiotemporal responsiveness to p53.

PubMed ID: 18510931
Article link: Cell

Species referenced: Xenopus laevis
Genes referenced: actl6a chrd.1 foxh1 foxh1.2 foxi1 hsp70 hspa1l mix1 nodal3.1 sall2 sia1 smad2 sox17a sox2 tbx2 tbxt tgfb1 tp53 tshz3 vegt ventx1.2 wnt8a XB5904899 [provisional] znf423 znf585b
Antibodies: Znf585b Ab1
Morpholinos: prdm9 MO1 tp53 MO1 znf585b MO1

Article Images: [+] show captions
References :
Piccolo, p53 regulation orchestrates the TGF-beta response. 2008, Pubmed, Xenbase